南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (03): 318-.

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Promoter methylation of Wilms’ tumor gene on the Xchromosome in gastric cancer

刘霞，王启明，牛会林，杨学习，孙静哲，张庆玲，丁彦青   

  1. 南方医科大学1南方医院病理科，2基础医学院病理系，3生物技术学院
  • 出版日期:2013-03-20 发布日期:2013-03-20

胃癌中新抑癌基因WTX启动子区域甲基化水平的检测

  1. 南方医科大学1南方医院病理科，2基础医学院病理系，3生物技术学院
  • Online:2013-03-20 Published:2013-03-20

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摘要： 目的探讨抑癌基因WTX启动子区域甲基化水平及其在胃癌中的作用。方法运用MassARRAY定量分析系统分析20例
胃癌及配对正常组织和3种胃癌细胞株（MGC803、SCG7901和BGC823）中WTX基因启动子区域甲基化水平。应用5-杂氮-2’-
脱氧胞苷(5-aza-dC）对胃癌细胞BGC823进行去甲基化处理，分析其对WTX基因启动子区域甲基化水平的影响。结果在胃癌
及其配对正常组织和胃癌细胞株中，WTX基因启动子区域甲基化水平普遍较低，并且3组之间无统计学差异。用5-aza-dC处理后
并不能改变胃癌细胞株中WTX基因启动子区域的甲基化水平。结论胃癌中基本不存在WTX基因启动子区域高甲基化状态。

Abstract: Objective To investigate the changes in methylation levels of the promoters of the tumor suppressor gene Wilms’
tumor gene on the X-chromosome (WTX) and its possible role in gastric cancer. Methods WTX promoter methylation levels
were detected in 20 pairs of specimens of gastric cancer and matched normal tissues and in 3 gastric cancer cell lines (MGC803,
SCG7901, and BGC823) using the Sequenom MassARRAY quantitative analysis system. The gastric cancer cell line BGC823
was treated with 5-aza-2’-deoxycytidine (5-aza-dC) for demethylation and the changes in the level of WTX promoter
methylation were investigated. Results WTX promoter methylation levels were very low and showed no significant
differences among normal gastric tissues, gastric cancer tissues and the 3 gastric cancer cell lines. In BGC823 cells, treatment
with 5-aza-dC did not obviously affect the promoter methylation levels of WTX. Conclusion High methylation levels of WTX
promoters are rare in gastric cancer.