南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (02): 282-.

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组蛋白乙酰化修饰调控流感病毒复制中间体dsRNA引起的IL-6激活表达

汤必奎，吴守伟，胡明洁，刘小粉   

  • 出版日期:2013-02-20 发布日期:2013-02-20

Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells

  • Online:2013-02-20 Published:2013-02-20

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摘要： 目的研究组蛋白乙酰化修饰在流感病毒复制中间体dsRNA引起IL-6 表达中的调控作用。方法以病毒复制中间体
dsRNA为诱导物，利用组蛋白去乙酰化酶（HDAC）抑制剂、DNA甲基转移酶（DNMT）抑制剂或HDAC siRNA分别处理A549细
胞，双荧光素酶报告系统分析IL-6启动子活性、RT-PCR检测mRNA转录水平以及ELISA检测蛋白表达。结果dsRNA可激活
IL-6启动子活性、增强mRNA转录水平和蛋白的分泌表达，用HDAC抑制剂TSA处理或HADC siRNA干扰均可显著增强IL-6
启动子活性和mRNA转录，同时，HDAC抑制剂TSA和DNA甲基转移酶抑制剂DAC在调控IL-6表达过程中无协同作用。结
论在流感病毒复制中间体dsRNA引起IL-6表达上调过程中受到组蛋白乙酰化修饰调控。

Abstract: Objective To investigate the role of histone acetylation in regulating influenza virus replicative intermediate
double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells. Methods A549 cells were treated with
influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small
interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6
mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively. Results
Influenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA
significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play
an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6
expression. Conclusion Influenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by
histone acetylation.