南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (02): 262-.

• • 上一篇    下一篇

实时荧光定量PCR检测转基因阿尔茨海默病小鼠脑组织中miRNAs的差异表达

田密，丁宇，侯德仁，邓炎尧，李维，奉夏露   

  • 出版日期:2013-02-20 发布日期:2013-02-20

Real-time PCR for detecting differential expressions of microRNAs in the brain of a transgenic mouse model of Alzheimer’s disease

  • Online:2013-02-20 Published:2013-02-20

PDF

1638

摘要： 目的通过实时荧光定量PCR 方法检测APPswe/PS △E9 双转基因小鼠和对照小鼠脑组织中miRNA-135a-5p、
miRNA-135a-2-3p、miRNA-298-5p、miRNA-466b-3p 和miR-669f-3p 的表达。方法选取6 月龄大小的APPswe/PS△E9 双转基
因小鼠为实验组，同月龄同种系的野生型小鼠C57为对照组，分别提取其脑组织总miRNA，应用实时荧光定量PCR方法检测两
组小鼠脑组织中miRNA-135a-5p、miRNA-135a-2-3p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达。结果上述5种
miRNAs在实验组与对照组小鼠中的相对表达量分别为0.73±0.27、1.08±0.58，2.47±6.15、1.65±0.67，0.72±0.14、1.31±0.73，0.57±
0.34、1.06 ± 0.35，0.63 ± 0.26、1.02 ± 0.18。其中有统计学意义的是miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p 和
miR-669f-3p（P≤0.05）。结论miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p 和miR-669f-3p 在APPswe/PS△E9 双转基因
小鼠中有差异表达，可能在阿尔茨海默病的发病过程中起着重要作用。

Abstract: Objective To detect the expression of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and
miR-669f-3p in the brain tissue of the APPswe/PS △E9 double transgenic mouse model of Alzhaimer’s disease using real-time
PCR. Methods Six-month-old APPswe/PS △E9 double transgenic mice and wild-type C57 mice of the same species were
examined for the expressions of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the
brain tissue using real-time PCR. Results The relative expression levels of the 5 miRNAs in the transgenic versus the wild-type
mice were 0.73±0.27 vs 1.08±0.58, 2.47±6.15 vs 1.65±0.67, 0.72±0.14 vs 1.31±0.73, 0.57±0.34 vs 1.06±0.35, and 0.63±0.26 vs 1.02±0.18,
respectively, showing significance differences in the expressions of miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p, and
miR-669f-3p between the two groups (P<0.05). Conclusion miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p
are expressed differentially in APPswe/PS △E9 double transgenic mice, suggesting their important roles in the pathogenesis of
Alzheimer disease.