南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (02): 258-.

• • 上一篇    下一篇

沉默Sam68对鼻咽癌5-8F细胞增殖的影响及其机制

杨彬，冯德辉   

  • 出版日期:2013-02-20 发布日期:2013-02-20

Effects of Sam68 gene silencing on proliferation of nasopharyngeal carcinoma cell line 5-8F and its possible molecular mechanism

  • Online:2013-02-20 Published:2013-02-20

PDF

1458

摘要： 目的探讨Sam68基因对鼻咽癌5-8F细胞增殖能力的影响及可能的分子机制。方法通过靶向沉默鼻咽癌5-8F细胞内源
性Sam68基因，研究细胞增殖能力的变化情况，并采用qRT-PCR和Western blotting实验方法检测Sam68、细胞周期相关蛋白及
其上游分子的表达情况。结果转染Sam68靶向siRNA的5-8F细胞中，Sam68蛋白和mRNA表达水平均明显降低。MTT、细胞
周期实验证实干扰Sam68后5-8F细胞的增殖能力受到抑制；Western blotting检测结果显示细胞周期相关蛋白Cyclin D1表达明
显下调，p27 表达上调，同时p-FOXO3a、p-Akt 和p-GSK-3β的表达明显下调，而总FOXO3a、Akt 和GSK-3β的表达则无明显变
化。结论Sam68可能通过激活Akt/FOXO3a信号通路对细胞增殖的相关分子进行调控，进而影响鼻咽癌细胞的增殖能力。

Abstract: Objective To observe the effect of Sam68 gene silencing on proliferation of nasopharyngeal carcinoma (NPC) cell line
5-8F and explore its possible molecular mechanism. Methods The NPC cell line 5-8F was transfected with a small interfering
RNA (siRNA) targeting Sam68 and the cell proliferation changes were observed. Quantitative RT-PCR and Western blotting
were used to examine the changes in the expressions of Sam68, cell cycle-related proteins, and some up-stream proteins in the
transfected cells. Results Transfection of 5-8F cells with Sam68-specific siRNA significantly lowered the mRNA and proteins
levels of Sam68, suppressed cell proliferation, decreased the expression of Cyclin D1, and increased the expression of p27. The
transfected cells showed obviously decreased expressions of p-FOXO3a, p-Akt and p-GSK-3β, but the expressions of FOXO3a,
Akt and GSK-3β were not obviously affected. Conclusion Sam68 modulates the proliferation of NPC cells probably by
activating Akt/FOXO3a pathway and regulating the cell proliferation-related molecules.