南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (02): 235-.

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CD99调控经典霍奇金淋巴瘤细胞株L428向B细胞方向分化

周新华，黄学平，黄作平，王志强，赵彤   

  • 出版日期:2013-02-20 发布日期:2013-02-20

CD99 regulates redifferentiation of classical Hodgkin’s lymphoma cell line L428 towards B cells

  • Online:2013-02-20 Published:2013-02-20

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摘要： 目的分析上调CD99基因对经典霍奇金淋巴瘤（cHL）细胞株L428形态和免疫表型的影响，探究CD99基因对H/RS细胞
发生发展的意义。方法鬼笔环肽染色和大细胞计数分析过表达CD99基因对L428细胞形态的影响；免疫细胞化学和流式细胞
检测分析上调CD99基因对L428细胞免疫表型的改变。结果CD99基因的过表达导致L428细胞变小、骨架发生重建，丢失了
cHL的诊断标记CD30 和CD15，重现B细胞表型PAX5、CD19、CD79α、BCL-6和CD10。结论CD99基因过表达调控L428细胞
向B细胞方向转化，提示cHL的B细胞表型的缺失很可能是CD99基因下调的结果。

Abstract: Objective To explore the effect of CD99 overexpression on the morphology and differentiation-related phenotypes of
classical Hodgkin’s lymphoma cell line L428 and investigate the role of CD99 gene in Hodgkin/Reed-Sternberg (HRS) cell
generation and transformation. Methods The effect of CD99 overexpression on the cell morphology was detected by HE
staining and phalloidin staining. Differentiation-related protein expressions were detected by immunocytochemistry and flow
cytometry after stable tansfection of CD99 gene in L428 cells. Results CD99 overexpression caused a decrease of the cell size
and reorganization of the actin cytoskeleton in L428 cells. Upregulation of CD99 led to the loss of classical Hodgkin’s
lymphoma diagnosis marker CD30 and CD15 and the restoration of the B-cell makers of PAX5, CD19, CD79α, BCL-6, and
CD10. Conclusion CD99 overexpression leads to redifferentiation of L428 cells towards B cells, suggesting that the loss of
B-cell phenotype in classical Hodgkin’s lymphoma is very likely a result of down-regulated CD99 expression.