南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (01): 22-.

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IFN-γ增强人γδT细胞杀伤骨肉瘤细胞的研究

李朝旭，唐际存，叶招明   

  • 出版日期:2013-01-20 发布日期:2013-01-20

Interferon-γ enhances human γδ T cell-mediated osteosarcoma cell killing in vitro

  • Online:2013-01-20 Published:2013-01-20

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摘要： 目的研究干扰素-γ（IFN-γ）增强骨肉瘤患者γδ T细胞对骨肉瘤杀伤作用的影响。方法以唑来磷酸法扩增骨肉瘤患者外
周血γδ T细胞。分别使用实时荧光定量聚合酶链反应和流式细胞术检测IFN-γ处理前后骨肉瘤细胞Fas的表达情况；LDH法检
测骨肉瘤患者γδ T细胞对骨肉瘤细胞的杀伤活性。结果IFN-γ对骨肉瘤细胞Fas的表达明显提高（P<0.01），骨肉瘤患者γδ T细
胞对骨肉瘤细胞的杀伤作用显著增强（P<0.01）。抗人FasL抗体对γδ T细胞杀伤OS细胞作用影响较小（P>0.05）；但能显著阻断
γδ T细胞杀伤IFN-γ预处理OS细胞的作用，差异均有统计学意义（P<0.01）。结论IFN-γ可增强骨肉瘤患者γδ T细胞的抗骨肉
瘤作用。

Abstract: Objective To investigate the cytotoxic effect of γδ T cells from osteosarcoma patients against interferon-γ
(IFN-γ)-treated osteosarcoma cells in vitro. Methods Human γδ T cells were amplified by zoledronate from peripheral blood
cells of osteosarcoma patients. The expression of Fas on the osteosarcoma cells were measured by flow cytometry and
quantitative real-time PCR analysis before and after IFN-γ treatment. The cytoxicity of γδ T cells against osteosarcoma cells
was evaluated with LDH assay. Results IFN-γ significantly enhanced the susceptibility of the osteosarcoma cell lines HOS and
U2OS to the cycotoxicity of γδ T cells from osteosarcoma patients (P<0.01). IFN-γ obviously up-regulated the expression of Fas
in HOS and U2OS cells (P<0.01). Anti-FasL mAb failed to inhibit the cytotoxicity of γδ T cells in untreated osteosarcoma targets
(P>0.05), but significantly impaired γδ T cell cytotoxicity in IFN-γ pre-treated osteosarcoma targets (P<0.01). Conclusion
IFN-γ can enhance the cytotoxic effect of human γδ T cells from osteosarcoma patients against osteosarcoma cells in vitro.