南方医科大学学报

南方医科大学学报 ›› 2013, Vol. 33 ›› Issue (01): 142-.

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NADPH氧化酶p22phox与非瓣膜性心衰左室重量指数的相关性分析

杨树国，姚宗芹，李敏，尤润生，王爱红，赵洪进   

  • 出版日期:2013-01-20 发布日期:2013-01-20

Correlation between left ventricular mass index and NADPH oxidase p22phox in patients with non-valvular chronic heart failure

  • Online:2013-01-20 Published:2013-01-20

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摘要： 目的研究非瓣膜性慢性心衰患者吞噬细胞NADPH氧化酶p22phox表达与左室重量指数的相关性，初步探讨该途径导
致的氧化应激在非瓣膜性慢性心衰左室重构中的作用。方法收集59例非瓣膜性慢性心衰患者作为心衰组，20例健康体检者
为健康对照组。心衰组（HF）患者根据有无左室肥厚(LVM)，分为单纯心衰组和心衰合并左室肥厚组。计算其体表面积（BSA）
及体质指数（BMI），抽取其空腹外周静脉血，收集吞噬细胞，提取总RNA，进行RT-PCR反应，得到p22phox的最终表达量。同时
进行血清高敏C反应蛋白（hs-CRP）、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇
（HDL-C）、尿素氮（BUN）、肌酐（CR）、尿酸（UA）测定。测量并记录左室舒张末期内径（LVEDd）、左室收缩末期内径（LVEDs）、
室间隔厚度（IVST）、左室后壁厚度（LVPWT）、EF值等参数，计算左室质量指数（LVMI）。结果心衰组p22phox表达水平（0.91±
0.37）较对照组（0.68±0.33）表达增高，差别有统计学意义（P=0.039，P<0.05）；心衰合并左室肥厚组（1.58±0.20）较单纯心衰组
p22phox水平（0.71±0.24）增高，差别有统计学意义（P=0.026，P<0.05）；p22phox与LVMI呈正相关关系（r=0.508，P<0.05）。结论
NADPH氧化酶p22phox与非瓣膜性心衰左室质量指数呈正相关性，在一定程度上可提示左室重构的水平。

Abstract: Objective To study the correlation between the expression levels of phagocytic NADPH oxidase p22phox subunit
and left ventricular mass index (LVMI) in patients with non-valvular chronic heart failure and explore the role of oxidative
stress caused by NADPH oxidase p22phox subunit in left ventricular remodeling. Methods Semi-quantitative RT-PCR was
used to examine the expression levels of phagocytic NADPH oxidase p22phox in 59 patients with non-valvular chronic heart
failure and 20 control subjects. All the subjects underwent ultrasonic cardiography to record their IVST, LVPWT, LVEDd,
LVEDs, and EF. Based on the calculated LVMI, the patients were divided into heart failure without LV hypertrophy (LVH)
group and heart failure with LVH group. Results The patients with heart failure showed significantly higher expression of
phagocytic NADPH oxidase p22phox than the control subjects (0.91±0.37 vs 0.68±0.33, P=0.039), and the patients with LVH had
significantly higher p22phox expression than those without LVH (1.58±0.20 vs 0.71±0.24, P=0.026). LVMI showed a positive
correlation with the expression of p22phox in these patients (r=0.508, P<0.05). Conclusion NADPH oxidase p22phox
expression level is positively correlated with LVMI and can be indicative of the level of left ventricular remodeling in patients
with non-valvular chronic heart failure.