南方医科大学学报

南方医科大学学报 ›› 2012, Vol. 32 ›› Issue (12): 1699-.

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祛风通络方对肾小球系膜细胞周期蛋白表达的影响

吴喜利，孙万森，叶冰玉，安鹏，石兴民，傅荣国，王竹，乔成林   

  • 出版日期:2012-12-20 发布日期:2012-12-20

Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells

  • Online:2012-12-20 Published:2012-12-20

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摘要： 目的通过不同检测方法观察祛风通络方对肾小球系膜细胞周期调节蛋白表达的影响，进一步探讨祛风通络方抑制肾小
球系膜细胞增殖的作用机制。方法以体外培养肾小球系膜细胞为研究对象，应用血清药理学方法，分别采用激光扫描共聚焦显
微镜及免疫组化法检测各组大鼠肾小球系膜细胞周期蛋白的表达变化。结果与正常组比较，LPS组肾小球系膜细胞周期蛋白
CyclinD1、CDK2、P21表达明显增强（P<0.01），P27蛋白表达明显减弱（P<0.01）；经过药物干预治疗，肾小球系膜细胞周期蛋白
Cyclin D1、CDK2、P21的表达均有不同程度减弱（P<0.05或P<0.01），而P27蛋白表达明显增强（P<0.01），且祛风通络方对
Cyclin D1、P21和P27蛋白表达的调节明显优于贝那普利（P<0.05或P<0.01）。结论祛风通络方抑制肾小球系膜细胞增殖的作
用机制可能与其下调细胞周期蛋白Cyclin D1、CDK2、P21和上调P27蛋白的表达有密切关系。

Abstract: ObjectiveTo observe the effects of Qufengtongluo Recipe (QFTLR) on the expressions of cell cycle regulatory
proteins in rat mesangial cells (MCs)in vitro and investigate the mechanism by which QFTLR inhibits MC proliferation.
MethodsUsing the methods of serum pharmacology, we studied the expressions of cell cycle regulatory proteins in rat MCs
treated with QFTLR by laser scanning confocal microscope and immunohistochemistry. ResultsCompared with the normal
control cells, the cells challenged with lipopolysaccharide (LPS) showed significantly enhanced expressions of cyclin D1,
CDK2, and P21(P<0.01) and obviously lowered protein expression of P27(P<0.01). Treatment of the LPS-challenged cells with
QFTLR and benazepril both resulted in significantly attenuated expressions of cyclin D1, CDK2, and P21and obvious increase
of P27expression (P<0.05orP<0.01), but QFTLR produced stronger effects than benazepril in regulating of cyclinD1, P21and
P27 protein expressions (P<0.05 or P<0.01). Conclusion QFTLR inhibits rat MC proliferationin vitro possibly by
down-regulating the cellular expressions of cyclin D1, CDK2, and P21and up-regulating the expression of P27protein.