南方医科大学学报

南方医科大学学报 ›› 2012, Vol. 32 ›› Issue (10): 1461-.

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多西他赛对雄激素依赖型及非依赖亚型前列腺癌细胞中C-jun与雄激素受体相互作用的影响

陈玢屾，徐亚文，徐啊白，刘春晓，郑少波，李虎林，许凯   

  • 出版日期:2012-10-20 发布日期:2012-10-20

Effect of docetaxel on C-jun and androgen receptor interaction in prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells

  • Online:2012-10-20 Published:2012-10-20

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摘要： 目的研究C-jun和雄激素受体（AR)在多西他赛处理的前列腺癌LNCaP细胞及其非雄激素依赖亚型LNCaP-bic细胞中的
相互作用。方法采用多西紫杉醇处理雄激素依赖型LNCaP前列腺癌细胞及其亚型雄激素非依赖型LNCaP-bic细胞，采用荧光
素酶分析检测AR及AP-1基因的表达，利用Western blotting，免疫沉淀方法分析C-jun以及AR的蛋白表达和相互作用。结果
经多西他赛处理后，LNCaP-bic以及其父代LNCaP细胞AR和C-jun在基因表达水平均得到增强。Western blotting提示
LNCaP-bic细胞中C-jun的磷酸化水平高于LNCaP细胞，同时其PSA蛋白表达也更强。免疫沉淀结果提示多西他赛使
LNCaP-bic细胞株中的AR和C-jun有更高的相关作用。结论多西他赛可以激活AR非配体依赖的转录功能，AR和C-jun之间
的相互作用可能影响多西他赛对前列腺癌的化疗结果。

Abstract: ObjectiveTo investigate the effect of docetaxel on the interaction between C-jun and androgen receptor (AR) in
prostate cancer LNCaP cells and its androgen-independence subtype LNCaP-bic cells.MethodsLNCaP and LNCaP-bic cells
were treated with docetaxel and the changes in AR and AP-1expression were evaluated using luciferase assay. Western
blotting and immunoprecipitation assay were employed to analyze the effects of docetaxel on the expressions of C-jun and AR
and their interaction.ResultsLuciferase assay showed that LNCaP and LNCaP-bic cells expressed higher levels of AR and
C-jun after docetaxel treatment. Docetaxel induced a higher level of p-c-jun expression in LNCaP-bic cells than in the parental
LNCaP cells. Western blotting showed a strong PSA protein expression in LNCaP-bic cell after docetaxel treatment. Docetaxel
caused a stronger interaction between AR and C-jun in LNCaP-bic cells.ConclusionDocetaxel activates ligand-independent
AR transcription, and the interaction between AR and C-jun may affect the outcome of docetaxel chemotherapy.