南方医科大学学报

南方医科大学学报 ›› 2012, Vol. 32 ›› Issue (10): 1440-.

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HSV-2 LAT过表达vero细胞中microRNA的表达谱变化

王颖，樊建勇，杨慧兰，陈剑云   

  • 出版日期:2012-10-20 发布日期:2012-10-20

Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression

  • Online:2012-10-20 Published:2012-10-20

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摘要： 目的分析过表达疱疹病毒Ⅱ型（HSV-2）潜伏相关转录子LAT后vero细胞microRNA表达谱的表达变化。方法通过化
学合成的方法获得LAT基因的全长序列。构建HSV-2LAT逆转录病毒表达载体pRetroQ- AcGFP1-C1，包装获得HSV-2LAT
基因过表达的逆转录病毒。利用microRNA芯片技术，分析感染逆转录病毒HSV-2LAT后vero细胞microRNA表达谱的变化，
得到因LAT基因过表达而产生变化的microRNA。结果通过microRNA芯片分析，逆转录病毒HSV-2LAT感染vero细胞后，
可引起hsa-miR-23a*、kshv-miR-K12-3、hsa-miR-943、hsa-miR-634、hsa-miR-12705种microRNA2倍以上上调；使hsa-miR-181a-2*、hsa-miR-450b-5p、hsa-miR-31、hsa-miR-24、kshv-miR-K12-12*5种microRNA2倍以上下调。结论LAT基因过表达后
可引起vero细胞microRNA表达谱的变化。

Abstract: ObjectiveTo investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2LAT
overexpression.MethodsThe full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ-AcGFP1-C1vector, and the recombinant retrovirus expressing HSV-2LAT was packaged. Using a microRNA microarray, the
microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.ResultsIn
Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression,5 microRNAs (hsa-miR-23a*,
kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5(hsa-miR-181a-2*, hsa-miR-450b-5p,
hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated.ConclusionThe expression of HSV-2LAT can induce
changes in microRNA expression profile in Vero cells.