南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1791-1793.

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鳖甲煎丸对H22荷瘤小鼠的抑瘤作用及对增殖细胞核抗原表达的影响

张绪慧； 陈达理； 罗荣城；   

  1. 南方医科大学南方医院肿瘤科； 南方医科大学中医药学院中医基础教研室； 南方医科大学南方医院肿瘤科 广东广州510515； 广东广州510515；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    广东省中医药管理局资助项目(102077)~~

Inhibitory effect of Biejiajianwan pills on tumor growth and proliferating cell nuclear antigen expression in mice bearing H22 cell-induced tumor

ZHANG Xu-hui1,CHEN Da-li2,LUO Rong-cheng1 Department of Oncology,Nanfang Hospital1,Department of Basic Theory of Traditional Chinese Medicine2,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学南方医院肿瘤科； 南方医科大学中医药学院中医基础教研室； 南方医科大学南方医院肿瘤科 广东广州510515； 广东广州510515；
  • Online:2006-12-20 Published:2006-12-20

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摘要： 目的研究鳖甲煎丸对H22荷瘤小鼠的抑瘤作用及机制探讨。方法建立H22荷瘤小鼠动物模型,随机分成4组:鳖甲煎丸高剂量组、鳖甲煎丸低剂量组、阴性对照组、环磷酰胺组。连续用药15d。第15天处死小鼠,计算胸腺指数、脾指数;剥离瘤块称重并计算抑瘤率;用免疫组织化学方法检测各组荷瘤小鼠肿瘤组织的增殖细胞核抗原的表达强度。结果鳖甲煎丸有较好的抑瘤作用;鳖甲煎丸对H22荷瘤小鼠肿瘤组织的增殖细胞核抗原有显著的抑制作用。结论鳖甲煎丸抑瘤作用机制之一可能是通过抑制肿瘤组织的增殖细胞核抗原来实现的。 更多还原

Abstract: Objective To study the anti-tumor effect of Biejiajianwan pills and explore its mechanism.Methods Mice bearing neoplasm induced by H22 cell inoculation were randomized into 4 groups,namely the negative control group,positive control group(with cyclophosphamide treatment),and high-and low-dose Biejiajianwan pill group.The tumor-bearing mice received corresponding treatments for 15 consecutive days,after which their thymuses and spleens were isolated and weighed to calculate the thymus and spleen indices.The tumor mass was also weighed and the tumor inhibition rate calculated.Immunohistochemistry was performed to evaluate the expression intensity of proliferating cell nuclear antigen(PCNA)in the tumor tissue.Results The pills significantly inhibited the growth of the implanted tumor and reduced the expression of PCNA in the tumor.Conclusion One of the possible mechanisms of the anti-tumor effects of Biejiajian pills might be associated with the inhibition of PCNA expression in the tumor.

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