南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1785-1788.

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饮食加硼治疗维甲酸诱导骨质疏松模型大鼠的效果观察

许鹏； 胡万彪； 郭雄； 张银刚； 李幼芬； 姚建锋； 蔡乾坤；   

  1. 西安市红十字会医院骨关节科； 陕西省横山县红十字会医院； 西安交通大学环境与疾病相关基因教育部重点实验室； 西安市红十字会医院骨关节科 陕西西安710054； 陕西西安710000； 陕西西安710054； 陕西西安710061；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    西安市科技攻关计划项目(GG04231)~~

Therapeutic effect of dietary boron supplement on retinoic acid-induced osteoporosis in rats

XU Peng1,HU Wan-biao2,GUO Xiong1,ZHANG Yin-gang3,LI You-fen3,YAO Jian-feng1,CAI Qian-kun1 1Department of Orthopedics,Xi’an Red Cross Hospital,Xi’an 710054,China;2Red Cross Hospital of Hengshan County,Hengshan 710000,China;3Key Laboratory of Environment and Genes Related Diseases of Ministry of Education,Xi’an Jiaotong University,Xi’an 710061,China   

  1. 西安市红十字会医院骨关节科； 陕西省横山县红十字会医院； 西安交通大学环境与疾病相关基因教育部重点实验室； 西安市红十字会医院骨关节科 陕西西安710054； 陕西西安710000； 陕西西安710054； 陕西西安710061；
  • Online:2006-12-20 Published:2006-12-20

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摘要： 目的观察饮食加硼治疗维甲酸诱导的骨质疏松模型大鼠的效果,为硼临床治疗骨质疏松症提供实验依据。方法SD大鼠32只,随机分为正常组(n=8)和骨质疏松模型组(n=24)。以维甲酸80mg.kg-1.d-1灌胃15d,诱导骨质疏松模型。模型复制成功后,骨质疏松模型组又随机分为无措施对照组(n=8)、饮食加硼治疗组(n=8)和雌二醇治疗组(n=8)。治疗30d后,测定大鼠血清钙、磷、硼含量,及碱性磷酸酶(AKP)和酸性磷酸酶(TRAP)活性,测量大鼠全身、腰椎和胫骨骨密度,并观察大鼠股骨形态计量学变化。结果四组大鼠血清Ca和P含量无显著性差异,而硼含量硼治疗组明显高于其它三组。对照组大鼠血清AKP和TRAP活性增加,股骨松质骨和皮质骨骨量减少,破骨细胞数量增加,腰椎、胫骨骨密度下降,呈现骨质疏松变化。饮食加硼和雌激素治疗组大鼠血清TRAP活性显著下降,股骨平均骨小梁数、平均骨小梁宽度、骨小梁面积百分比、皮质骨面积百分比和全身、腰椎、胫骨骨密度等显著增加,松质骨区破骨细胞数明显减少,与正常组大鼠骨质无明显差异。硼治疗组大鼠血清AKP活性及松质骨区活跃成骨细胞数显著增加。结论饮食加硼可提高大鼠血清硼含量,增加骨形成,减少骨吸收,对骨质疏松有明显的治疗作用。

Abstract: Objective To observe the therapeutic efficacy of dietary boron supplement on retinoic acid-induced osteoporosis in rats,so as to provide experimental evidence for clinical management of osteoporosis with boron.Methods Thirty-two SD rats were randomized into normal control group(8 rats)and osteoporotic group(24 rats),and osteoporosis was induced in rats of the latter group by intragastric retinoic acid administration at the daily dose of 80 mg/kg for 15 consecutive days.The osteoporotic rats were subdivided into control group(8 rats)without treatment,boron treatment group(8 rats)and estradiol treatment group(8 rats).After 30 days of treatment,the serum contents of Ca,P,boron and the activities of alkaline phosphatase(AKP)and tartrate-resistant acid phosphatase(TRAP)in the rats were assayed,the bone mineral density(BMD)of the whole body,lumbar vertebrae and tibia were determined,and the morphological changes of the femurs were observed.Results The serum contents of Ca and P in the rats of the 4 groups differed scarcely,but the content of boron in boron treatment group was markedly higher than that in the other three groups.In the osteoporotic control group,the activities of serum AKP and TRAP,the masses of spongy bone and cortical bone of the femurs,and the quantity of the osteoclasts were increased,with the BMD of the lumbar vertebrae and tibia decreased,suggesting osteoporotic conditions.The mean trabecular plate density and thickness,trabecular bone volume and cortical bone volume of the femurs in the osteoporotic rats treated with boron or estradiol were significantly increased,but the active osteoclast quantity in the spongy bone and serum TRAP activities were obviously decreased,and the bone quality was comparable with that of the normal group.In addition,the serum AKP activity and the active osteoblast quantity in the spongy bone were obviously increased in boron treatment group.Conclusion The dietary boron supplement can increase the serum content of boron of osteoporotic rats to stimulate bone formation and inhibit bone resorption,producing therefore obvious therapeutical effect against osteoporosis.

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