南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1757-1759.

• • 上一篇    下一篇

胎鼠肝nestin阳性细胞的分离培养和诱导分化研究

刘军； 安靓； 陈金拳；   

  1. 南方医科大学组织与胚胎学教研室； 南方医科大学组织与胚胎学教研室 广东广州510515； 广东广州510515；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    广东省自然科学基金(2004B34001007)~~

Isolation,culture,and differentiation induction of nestin-positive cells in fetal rat hepatic cells

LIU Jun,An Jing,CEHN Jin-quan Department of Histology and Embryology,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学组织与胚胎学教研室； 南方医科大学组织与胚胎学教研室 广东广州510515； 广东广州510515；
  • Online:2006-12-20 Published:2006-12-20

PDF

1226

摘要： 目的建立胎鼠肝nestin阳性细胞的体外分离、培养和诱导分化的方法。方法采用机械分离法结合悬滴培养法获得胎肝干细胞,原代培养2d后改变培养基,添加含20%胎牛血清的DMEM(含25ml/L葡萄糖,100U/ml青链霉素,pH值7.6),添加烟酰胺10mmol/L,胰岛素1mg/L,N2添加剂,碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。结果和结论大鼠胎肝内存在nestin阳性细胞,在体外扩增后加入诱导剂可定向分化为胰岛β细胞。 更多还原

Abstract: Objective To establish the method for isolation,culture,and differentiation induction of nestin-positive cells in fetal rat hepatic cells.Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture,and after two days of primary culture,the medium was changed for further cell culture in the presence of 20% fetal bovine serum(containing glucose 25 ml/L,mycillin 100 U/ml,pH 7.6),10 mmol/L nicotinamide,1 mg/L insulin,affix N2,basic fibroblast growth factor,stem cell factor,epidermal growth factor and leukemia inhibitory factor.Result and conclusion Nestin-positive cells were obtained from fetal rat liver,which can differentiate into islet β cells after culture and expansion in vitro.

中图分类号: