南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1742-1744.

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氯沙坦对糖尿病大鼠肾组织血管紧张素Ⅱ及其1型受体mRNA表达的影响

吴文； 丁鹤林； 陈黎红； 傅祖植；   

  1. 广东省人民医院老年病研究所内分泌科； 中山大学附属第二医院内分泌科； 中山大学附属第二医院内分泌科 广东广州510080； 广东广州510120；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    广东省自然科学基金(A2004004)~~

Effects of losartan on the levels of angiotensinⅡ and its type-1 receptor in diabetic rat kidney

WU Wen1,DING He-lin2,CHEN Li-hong2,FU Zu-zhi2 1Department of Endocrinology,Institute of Geriatrics,People’s Hospital of Guangdong Province,Guangzhou 510080,China;2Department of Endocrinology,Second Hospital of Sun Yat-sen University,Guangzhou 510120,China   

  1. 广东省人民医院老年病研究所内分泌科； 中山大学附属第二医院内分泌科； 中山大学附属第二医院内分泌科 广东广州510080； 广东广州510120；
  • Online:2006-12-20 Published:2006-12-20

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摘要： 目的研究氯沙坦对实验性糖尿病大鼠肾组织血管紧张素Ⅱ(AngⅡ)1型受体mRNA表达的影响。方法纯种雄性Wistar大鼠分为3组,A组(n=11)为正常对照组,B组(n=11)为糖尿病为干预组,C组(n=9)为糖尿病大鼠氯沙坦干预组。以链脲佐菌素制备糖尿病大鼠模型。饲养18周后取出肾脏检测AngⅡ水平、AngⅡ1型受体mRNA表达,收集24h尿测定尿白蛋白排泄及肌酐并心脏内取血检测血AngⅡ及肌酐。AngⅡ1型受体mRNA表达采用RT-PCR,以-actin作为内对照。尿白蛋白排泄采用大鼠白蛋白特异的酶免疫分析试剂盒。结果血、肾组织AngⅡ在A、B、C三组间无显著性差异。肾组织AngⅡ1型受体mRNA表达在B组大鼠(0.62±0.17)显著低于A组(1.13±0.82,P<0.01)及C组(1.13±0.62,P<0.01)。尿白蛋白排泄在B组大鼠(2.18±1.98mg/d)显著高于A组(0.41±0.47mg/d,P<0.01)及C组(0.65±0.89mg/d,P<0.01)。结论氯沙坦处理能升高糖尿病大鼠肾组织的AngⅡ1型受体mRNA表达,但不改变血液及肾组织AngⅡ水平。

Abstract: Objective To investigate the effect of losartan,an angiotensin II type-1 receptor(AT1R)antagonist,on the levels of angiotensinⅡ(AngⅡ)and AT1R in diabetic rat kidney.Methods Male Wistar rats were divided into 3 groups,group A(n=11)served as the control group,group B(n=11)included the diabetic rats(induced by intraperitoneal injection of streptozotocin)without any therapy,and group C(n=9)diabetic rats treated with losartan.After 18 weeks of treatment,the kidneys were taken from all the rats to measure the expression of AT1R mRNA by RT-PCR and detect the AngⅡ level.Blood was also drawn from the heart to measure AngⅡ level,and 24-hour urine was collected to measure albumin level(urine albumin excretion,UAE)with rat albumin enzyme immunoassay kit.Results The blood and renal AngⅡ levels showed no significant difference between the 3 groups.The expression of renal AT1R mRNA in group B(0.62±0.17)was significantly lower than that in group A(1.13±0.82,P<0.01)and group C(1.13±0.62,P<0.01).UAE in group B(2.18±1.98 mg)was significantly higher than that in group A(0.41±0.47 mg/d,P<0.01)and C(0.65 ±0.89 mg/d,P<0.01).Conclusion Losartan can increase the expression of AT1R mRNA in diabetic rat kidneys without altering the blood and renal AngⅡ levels.

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