南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1728-1731.

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Sonic hedgehog信号通路Smo蛋白及其下游转录因子Gli1蛋白在胃癌组织中的表达及其意义

戎祯祥； 方驰华； 朱达坚； 刘胜军；   

  1. 南方医科大学珠江医院普通外科； 南方医科大学珠江医院普通外科 广东广州510282； 广东广州510282；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    广东省自然科学基金(010597,020093)~~

Expression of Smo protein and the downstream transcription factor Gli1 protein in Sonic hedgehog signal transduction pathway in gastric carcinoma

RONG Zhen-xiang,FANG Chi-hua,ZHU Da-jian,LIU Sheng-jun Department of Hepatobiliary Surgery,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China   

  1. 南方医科大学珠江医院普通外科； 南方医科大学珠江医院普通外科 广东广州510282； 广东广州510282；
  • Online:2006-12-20 Published:2006-12-20

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摘要： 目的探讨Sonichedgehog信号通路Smo蛋白及其下游转录因子Gli1蛋白在胃癌组织中的表达及其意义。方法收集85例胃癌组织及25例正常胃粘膜组织构建成组织芯片,应用免疫组化S-P法检测胃癌组织中Smo蛋白及Gli1蛋白的表达,并以正常胃粘膜组织作对照,分析两者的相关性。结果Smo和Gli1蛋白均在正常胃粘膜中不表达或弱表达;在胃乳头状腺癌、管状腺癌、低分化腺癌中,两者的表达强度和阳性表达率显著高于正常胃粘膜(P<0.05,并随着胃腺癌分化程度下降而上升;相关分析显示Smo和Gli1蛋白在胃癌组织中的表达呈正相关,相关系数为0.989,P<0.001。结论胃癌发生过程中的Sonichedgehog信号通路异常激活可能通过Smo蛋白高表达上调下游转录因子Gli1蛋白的表达参与部分胃腺癌的发生。 更多还原

Abstract: Objective To study the expression of Smo protein and the downstream transcription factor Gli1 protein in Sonic hedgehog signal transduction pathway in gastric carcinoma.Methods A tissue microarray was constructed using 85 gastric carcinoma and 25 normal gastric mucosa specimens.The expression of Smo and Gli1 proteins were detected immunohistochemically and the correlation between their expression in gastric carcinoma was analyzed.Results Only weak expression,if any,of Smo and Gli1 proteins was detected in normal gastric mucosa,but in papillary adenocarcinoma,tubular adenocarcinoma and poorly differentiated adenocarcinoma,their expressions were significant increased as the differentiation degree was lowered.Smo protein expression in gastric carcinoma was significantly correlated with that of Gli1 protein with correlation coefficient of 0.989(P<0.001).Conclusions The abnormal activity of Sonic hedgehog signal transduction pathway may play an important role in the occurrence of papillary adenocarcinoma,tubular adenocarcinoma and poorly differentiated adenocarcinoma,and this abnormality is associated with Smo protein overexpression,which upregulates the expression of the downstream transcription factor Gli1 protein.

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