南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1706-1708.

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全氟丙烷人血白蛋白微球注射剂静脉给药后试验犬气体采集方法的建立

杨凌； 陈志良； 刘世霆； 杨莉；   

  1. 南方医科大学南方医院药材科； 南方医科大学南方医院药材科 广东广州510515； 广东广州510515；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    国家重点科技攻关项目(969010588)~~

Sampling of exhaled gas after intravenous administration of octafluoropropane-containing human albumin micropheres in dogs

YANG Ling,CHEN Zhi-liang,LIU Shi-ting,YANG Li Department of Pharmacy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学南方医院药材科； 南方医科大学南方医院药材科 广东广州510515； 广东广州510515；
  • Online:2006-12-20 Published:2006-12-20

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摘要： 目的建立一种新型全氟丙烷人血白蛋白微球注射剂静脉给药后定时、定量采集试验犬呼出气体的方法,为该药的药代动力学研究奠定基础。方法试验犬在麻醉、呼吸机通气状态下,定时、定量采集其呼出气体样本。采用气相色谱——二级质谱法测定样本浓度。结果试验犬呼出气体中全氟丙烷的AUC与注射剂量基本呈线性关系,Y=1162.5X-417.38(r2=0.9499)。全氟丙烷总体回收率为(119.49±27.62)%,与回收率100%比较,P>0.05,无显著性差异;全氟丙烷累积百分率个体差异较大。GC-MS-MS方法测定全氟丙烷气体,准确、灵敏、精确,能充分满足实验需要。结论该方法能反应全氟丙烷气体在试验犬体内的药代动力学特征,可用于全氟丙烷或包含其他气体药物的药代动力学研究。 更多还原

Abstract: Objective To develop a method for quantitative collection of exhaled gas in anesthetized dogs at given time following intravenous administration of octafluoropropane(OFP)-containing human albumin micropheres for assessing the gas kinetics of OFP.Methods OFP-containing albumin micropheres were administered intravenously at 0.4,0.8 and 1.2 ml/kg,respectively,in anesthetized and ventilated dogs.The exhaled air samples were analyzed by gas chromatography-tandem mass spectrometry(GC-MS-MS).Results The correlation curve between the area under curve(AUC)and administered dose was roughly linear(Y=1162.5X-417.38,r2=0.949 9).The total recovery rate was(119.49±27.62)% which was not significantly different from the rate of 100%(P>0.05).GC-MS-MS was accurate,sensitive,precise and applicable for OFP determination.Conclusion The sampling method is useful for characterizing OFP pharmacokinetics in dogs,and also applicable for studying the pharmacokinetics of other gas-containing drugs.

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