南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (12): 1698-.

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Slingshot-1L在哮喘病人外周血嗜酸粒细胞的表达及其功能

张卫珍； 蔡绍曦； 邹飞； 赵海金； 李文军；   

  1. 南方医科大学南方医院呼吸科； 南方医科大学公共卫生和热带医学学院； 南方医科大学公共卫生和热带医学学院 广东广州510515； 广东广州510515；
  • 出版日期:2006-12-20 发布日期:2006-12-20
  • 基金资助:
    国家自然科学基金(30270593);广东省自然科学基金(04105757)~~

Role of Slingshot-1L in peripheral eosinophils of asthmatic patients

ZHANG Wei-zhen1,CAI Shao-xi1,ZOU Fei2,ZHAO Hai-jin1,LI Wen-jun2 Department of Respiratory Diseases,Nanfang Hospital1,College of Public Health and Tropical Medicine2,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学南方医院呼吸科； 南方医科大学公共卫生和热带医学学院； 南方医科大学公共卫生和热带医学学院 广东广州510515； 广东广州510515；
  • Online:2006-12-20 Published:2006-12-20

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摘要： 目的探讨Slingshot-1L(SSH-1L)在哮喘病人外周血嗜酸粒细胞(EOS)的表达及其功能。方法抽取15例正常自愿者(正常组)及30例哮喘病人(未经糖皮质激素治疗组15例,已予糖皮质激素治疗15例)外周静脉血各30ml进行EOS的分离纯化并计数,提取其总RNA及总蛋白进行特异性片段SSH-1L的RT-PCR扩增及Westernblotting,从基因和蛋白水平比较不同组别之间SSH-1L表达的变化。结果未治疗组EOS的SSH-1L/β-Actin灰度值比与正常组及治疗组比较均显著升高(P<0.05),治疗组EOS的SSH-1L/β-Actin灰度值比与正常组比较无显著差异(P>0.05),未治疗组蛋白样品SSH-1L光密度值(Dλ值)比正常组及治疗组显著升高(P<0.05),正常组蛋白样品SSH-1LOD值与治疗组比较无显著差异(P>0.05)。结论SSH-1L在急性发作期哮喘病人外周血EOS表达明显增高,可能在EOS激活、迁移上发挥重要作用。

Abstract: Objective To investigate the expression of Slingshot-1L(SSH-1L)in peripheral blood eosinophils of asthmatic patients.Methods Peripheral vein blood sample of 30 ml was collected from 15 healthy volunteers,15 asthmatic patients with glucocorticoid treatment and 15 asthmatic patients without the treatment.The eosinophils were isolated,purified and counted for each sample,and SSH-1L/β-actin gene fragments were amplified simultaneously by RT-PCR for the total RNA.SSH-1L protein was detected by Western blotting from the total protein of the peripheral eosinophils.The expressions of SSH-1L at both mRNA and protein levels are compared between different groups.Results SSH-1L/β-actin ratio significantly increased in untreated patients with asthma attacks in comparison with healthy volunteers(P<0.05),which did not occur in patients treated with glucocorticoids(P>0.05).The optical density of SSH-1L protein significantly increased in untreated asthmatic patients(P<0.05),but not in patients treated with glucocorticoids(P>0.05),as compared with the healthy volunteers.Conclusion Significantly increased SSH-1L expression in peripheral eosinophils may play an important role in the activation and migration of eosinophils.

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