南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (11): 1633-1636.

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钙调磷酸酶Aα基因过表达在缺血/再灌注和肾上腺素能受体介导的心肌凋亡中的作用

沈小梅； 张巨艳； 成蓓；   

  1. 华中科技大学同济医学院协和医院心血管内科； 山西医科大学第一附属医院； 华中科技大学同济医学院协和医院心血管内科 湖北武汉430022山西医科大学第一附属医院； 山西太原030001； 湖北武汉430022；
  • 出版日期:2006-11-20 发布日期:2006-11-20

Effect of calcineurin Aα gene overexpression on the myocardium apoptosis induced by hypoxia-reoxygenation and adrenergic receptors

SHEN Xiao-mei1,2,ZHANG Ju-yan2,CHENG Bei1 1Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;2First Affiliated Hospital,Shanxi Medical University,Taiyuan 030001,China   

  1. 华中科技大学同济医学院协和医院心血管内科； 山西医科大学第一附属医院； 华中科技大学同济医学院协和医院心血管内科 湖北武汉430022山西医科大学第一附属医院； 山西太原030001； 湖北武汉430022；
  • Online:2006-11-20 Published:2006-11-20

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摘要： 目的研究腺病毒介导钙调磷酸酶Aα基因(AdCnAα)过表达对缺血/再灌注和肾上腺素能受体介导的心肌凋亡中的作用及p38MAPK的变化。方法体外培养乳鼠心肌细胞,分别给予Iso(10μmol/L)+缺氧24h/复氧4h(Iso+H/R)及感染重组腺病毒(AdCnAα)+Iso+H/R处理,观察AdCnAα对Iso+H/R诱导的心肌细胞凋亡的影响。用DNALadder法及流式细胞仪DNA含量测定法(PI标记)检测心肌细胞凋亡,用RT-PCR法和Westernblot法分别检测CaNmRNA及蛋白表达,用Westernblot法检测p38、p-p38MAPK的变化。结果CnAα过表达组(AdCnAα+Iso+H/R)的心肌细胞凋亡率比Iso+H/R组明显增加(14.247±0.525vs10.763±1.554,P<0.01),且均高于正常对照组;同时p-p38MAPK的表达也比正常对照组增加(2.42±0.19vs1.60±0.22,P<0.01);p38MAPK的表达在干预前后同正常对照组比较无差异性变化(P>0.05)。结论腺病毒介导的CnAα过表达可使Iso+H/R共同作用诱导的心肌细胞凋亡率增加,p38MAPK可能参与了CaN促进Iso+H/R诱导心肌细胞凋亡的信号转导过程。

Abstract: Objective To investigate the effect of calcineurin AαcDNA(AdCnAα) overexpression as a result of adenovirally mediated gene transfer on neonatal rat cardiac myocyte apoptosis induced by hypoxia-reoxygenation(H/R) and adrenergic receptors.Methods Neonatal rat cardiac myocytes were cultured for 20 h after AdCnAα transfection,and treated with isoproterenol(10 μmol/L) and 24 h of hypoxia followed by 4 h of reoxygenation(24H/4R).The cardiac myocyte apoptosis induced by the treatments was assessed by flow cytometry and DNA laddering,and the levels of calcineurin,p38 and phosphorylation p38(p-p38) were determined by Western blotting and(or) RT-PCR.Results AdCnAα transfection promoted cultured neonatal rat cardiac myocyte apoptosis induced by isoproterenol+24H/4R as compared with the treated cells without transfection(14.247±0.525 vs 10.763±1.554,P<0.01),along with greater phosphorylation p38 protein expression(1.60±0.22 vs 2.42±0.19,P<0.01).The levels of p38 underwent no obvious change after AdCnAα transfection(P<0.05).Conclusions AdCnAα transfection can promote cardiac myocyte apoptosis induced by H/R and adrenergic receptors,the mechanism of which might be associated with p38 mitongen-activated protein kinase(p38MAPK) activation. 

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