南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (10): 1510-1512.

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改良反相高效液相色谱法测定血清中异丙酚浓度

范莹盈； 徐礼鲜； 文爱东； 张惠； 刘春然； 李薇； 每晓鹏；   

  1. 第四军医大学口腔医院麻醉科； 第四军医大学西京医院药剂科； 第四军医大学口腔医院麻醉科 陕西西安710032； 陕西西安710032；
  • 出版日期:2006-10-20 发布日期:2006-10-20

Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection

FAN Ying-ying1, XU Li-xian1, WEN Ai-dong2, ZHANG Hui1, LIU Chun-ran1, LI Wei2, MEI Xiao-peng1 Department of Anesthesiology, Stomatology Hospital1, Department of Pharmacy, Xijing Hospital2, Fourth Military Medical University, Xi’an 710033, China   

  1. 第四军医大学口腔医院麻醉科； 第四军医大学西京医院药剂科； 第四军医大学口腔医院麻醉科 陕西西安710032； 陕西西安710032；
  • Online:2006-10-20 Published:2006-10-20

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摘要： 目的创建一种新型反相高效液相色谱-荧光法测定血清中异丙酚浓度,为临床上快速准确的监测异丙酚血药浓度提供科学实用的检测手段。方法应用20%高氯酸沉淀血清样品后,混匀离心取上清液50μL进样分析。分析柱为DiamonsilC18(250mm×4.6mm,5μm),流动相为乙腈-水(90∶10)(冰醋酸调pH=4.0),流速1.0mL/min,荧光激发波长为276nm,发射波长为310nm。柱温为室温。结果当测定浓度范围为0.05-10μg/mL时,异丙酚与内标麝香草酚的峰面积之比与浓度呈良好的线性关系(r=0.9975),最低检出浓度50ng/mL,日内与日间精密度在4.78-6.59之间,明显小于15%。结论改良反相高效液相色谱法测定人血清异丙酚浓度具有准确高效、灵敏快捷等特点,适用于科研和临床异丙酚血药浓度的监测。R

Abstract: Objective To develop a new high-performance liquid chromatographic (HLPC) method for determination of propofol in human serum. Methods Human serum samples were precipitated with 20% perchloric acid and centrifuged to obtain 50 μl of the supernatant for analysis by HPLC coupled with fluorescence detection. The analysis was performed with a C18 reversed-phase column using a acetonitrile-water (90∶10) phase delivered at 1.0 ml/min, with the excitation wavelength of 276 nm and emission wavelength of 310 nm. Results The calibration curves were linear (r=0.997 5) within the concentration range of 0.05-10 μg/ml, the limit of propofol quantification was 50 ng/ml and the intra- and inter-day precisions were between ±15%. Conclusion The method is accurate, sensitive and simple for propofol determination in clinical anesthesia. 

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