南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (10): 1460-1463.

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9种柴胡属植物的核糖体ITS序列及其在药材鉴定中的应用

谢晖； 晁志； 霍克克； 吴炳义； 潘胜利；   

  1. 复旦大学药学院生药教研室； 南方医科大学中药学院； 复旦大学生命科学研究院； 南方医科大学南方医院临床实验研究中心； 复旦大学药学院生药教研室 上海200321； 广东广州510515； 上海； 200433； 上海200321；
  • 出版日期:2006-10-20 发布日期:2006-10-20
  • 基金资助:
    广州市科技计划(2002J1-C0161)~~

ITS sequence of 9 Bupleurum species and its application in identification of Chaihu (Radix Buplurei)

XIE Hui1, CHAO Zhi2, HUO Ke-ke3, WU Bing-yi4, PAN Sheng-li1 1School of Pharmacy, Fudan University, Shanghai 200032, China; 2School of Tradition Chinese Medicine, Southern Medical University, Guangzhou 510515, China; 3Institute of Biological Science, Fudan University, Shanghai 200433, China; 4Clinical Research Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 复旦大学药学院生药教研室； 南方医科大学中药学院； 复旦大学生命科学研究院； 南方医科大学南方医院临床实验研究中心； 复旦大学药学院生药教研室 上海200321； 广东广州510515； 上海； 200433； 上海200321；
  • Online:2006-10-20 Published:2006-10-20

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摘要： 目的研究9种柴胡属药用植物rDNAITS序列的差异和规律,寻找可准确鉴定柴胡类药材的分子性状。方法PCR扩增ITS序列,测序,DNAssistversion2.2软件进行序列比对,并计算同源性。结果柴胡属植物ITS序列与外类群ITS序列比对同源性均小于75%,序列间两两比对同源性均大于88%,同种植物则大于99%。结论ITS序列作为柴胡类药材鉴定依据具有一定实用性和可靠性。 

Abstract: Objective The study the patterns of rDNA ITS sequence variation of 9 medicinal species of genus Bupleurum in China to find the DNA molecular markers for identification of these crude drugs. Method The ITS regions of these species were amplified by PCR and sequenced, and their sequences were analyzed by DNAssist Version 2.2. Results Homologous alignment indicated that the consanguinity of the ITS sequences between genus Bupleurum and outgroups was lower than 75%, and that within genus Bupleurum was higher than 88%. For the same species, the consanguinity was higher than 99%. Conclusion The ITS sequences may serve as reliable molecular markers for identification of Radix Bupleuri. 

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