南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (10): 1421-.

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IgA肾病内皮源型一氧化氮合酶基因多态性分析

洪权； 丁瑞； 陈香美； 吕杨；   

  1. 解放军总医院肾病中心暨肾病重点实验室； 解放军总医院肾病中心暨肾病重点实验室 北京100853； 北京100853；
  • 出版日期:2006-10-20 发布日期:2006-10-20
  • 基金资助:
    国家自然科学基金“创新研究群体”项目(30121005)~~

Association between endothelial nitric oxide synthase gene 4a/b polymorphism and IgA nephropathy

HONG Quan, DING Rui, CHEN Xiang-mei, Lü Yang Kidney Center and Key Laboratory of PLA, General Hospital of PLA, Beijing 100853, China   

  1. 解放军总医院肾病中心暨肾病重点实验室； 解放军总医院肾病中心暨肾病重点实验室 北京100853； 北京100853；
  • Online:2006-10-20 Published:2006-10-20

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摘要： 目的研究内皮型NO合酶(eNOS)基因多态性与IgA肾病的关系。方法采用聚合酶链反应法对北方地区(汉族:北京,天津,河北)296例IgA患者、310例正常对照两组人群的eNOS基因第4内含子的可变串联重复序列多态性(VNTR)4a/b多态性进行基因型分析,比较两组间基因频率、等位基因频率分布有无统计学差异。结果两组患者的eNOS基因的可变串联复序列表现为eNOS4a/4a,eNOS4b/4b,eNOS4a/4b3种基因型。IgA肾病患者组和正常对照组之间的eNOS基因4a/b的基因型和等位基因频率无显著性差异。结论eNOS基因内含子4VNTR多态性与IgA肾病血管病变的发生无明显关联。该多态性在IgA肾病遗传因素中的地位尚难以确定,其参与IgA肾病微血管病变的作用可能存在种族或地域异质性。 

Abstract: Objective To investigate the relation between endothelial nitric oxide synthase (eNOS) gene polymorphism and IgA nephropathy (IgAN). Methods The variable number of tandem repeats (VNTR) polymorphism of eNOS gene was analyzed with PCR in 296 Chinese Han patients with IgAN groups and 310 healthy Han subjects from Beijing, Tianjin and Hebei Province, and the gene frquency and allele frequency were compared between the two groups. Results In these Chinese Han subjects involved in this study, VNTR of eNOS gene showed 3 genetypes, namely eNOS4a, eNOS4b and eNOS4a/4b. No significant difference was found in the gene frequency between IgAN group and the healthy subjects. Conclusions IgAN is not associated with VNTR polymorphism of eNOS gene, and no sufficient evidence has been obtained to establish the relation of eNOS VNTR polymorphism with the genetic factors of IgAN. The role of eNOS gene polymorphism in IgAN can be heterogenous in regard to different races or areas. 

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