摘要: 目的构建四环素诱导性鼠Smad7真核表达载体。方法采用常规分子生物学方法,抽提SD大鼠肾脏总RNA,RT-PCR获得鼠Smad7cDNA,再定向克隆于四环素诱导性真核表达载体pBI-L多克隆位点NheI和HindIII之间,限制性内切酶酶切和测序鉴定。结果重组pBI-L-Smad7真核表达载体经限制性内切酶酶切分析和测序分析,与理论值相符。结论本实验成功构建鼠Smad7四环素诱导性真核表达载体pBI-L-Smad7,为今后临床开展组织器官纤维化Smad7基因治疗奠定实验基础。
中图分类号:
任淑婷; 于琳华; 徐长福; 高广道;. 诱导性鼠Smad7真核表达载体的构建与鉴定[J]. 南方医科大学学报, 2006, 26(09): 1313-1315.
REN Shu-ting1, YU Lin-hua1, XU Chang-fu1, GAO Guang-dao2 Department of Pathology1, Department of Physiology and Pathophysiology2, Medical College of Xi’an Jiaotong University, Xi’an 710061, China. Construction and identification of tetracycline-inducible rat Smad7 eukaryotic expression vector[J]. Journal of Southern Medical University, 2006, 26(09): 1313-1315.