南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1255-1257.

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TGFα对胃癌细胞cyclin E和cyclin D1表达的影响

梁卫江； 张万岱； 陈村龙； 姚永莉； 罗荣城；   

  1. 南方医科大学南方医院肿瘤中心； 南方医科大学南方医院消化内科； 南方医科大学南方医院肿瘤中心 广东广州510515； 广东广州510515；
  • 出版日期:2006-08-20 发布日期:2006-08-20

Effect of transforming growth factor α on the expression of cyclin E and cyclin D1 in gastric carcinoma cells

LIANG Wei-jiang1, ZHANG Wan-dai2, CHEN Cun-long2, YAO Yong-li2, LUO Rong-cheng1 1Cancer Center, 2Department of Digestive Disease, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学南方医院肿瘤中心； 南方医科大学南方医院消化内科； 南方医科大学南方医院肿瘤中心 广东广州510515； 广东广州510515；
  • Online:2006-08-20 Published:2006-08-20

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摘要： 目的探讨TGFα对胃癌细胞cyclinE和D1表达的促进作用,为研究TGFα促胃癌发生发展机制提供重要信息。方法①细胞体外培养,用无血清RPMI-1640阻断法进行G0/G1期同步化,用碘化丙啶染色和流式细胞术,检测其同步化率;②细胞经G0/G1期同步化后,给予2.5%低浓度血清+RPMI-1640培养处理,以及在此基础上分别加入10、30、50μg/LTGFα处理,均作用5h;用免疫荧光染色及流式细胞术,检测细胞cyclinE和D1的表达情况。结果细胞经无血清RPMI-1640阻断法处理,其G0/G1期的百分率由常规培养的54%上升到72%;加入TGFα处理后,细胞cyclinE和D1表达均显著高于单用低血清培养(均P<0.001),并随TGFα剂量的增加而增加;当TGFα浓度为50μg/L时,两者的表达与单用低血清培养比,分别增加了25.18%和27.52%(均P<0.001)。结论TGFα可显著促进胃癌细胞cyclinE和D1的表达,从而发挥其促细胞周期进程的作用。

Abstract: Objective To explore the effect of transforming growth factor α (TGFα) on the expression of cyclin E and D1 in gastric carcinoma cells. Methods Human gastric adenocarcinoma SGC7901 cells were cultured routinely and synchronized at G0/G1 phase in serum-free RPMI-1640. The percentage of the cells at G0/G1 phase was detected by propidium iodide staining and flow cytometry (FCM), and the synchronized cells were cultured in RPMI-1640 supplemented with 2.5% calf serum and treated with 10, 30, and 50 μg/L TGFα for 5 h. The expression of cyclin E and D1 in SGC7901 cells was detected by immunofluorescent staining and FCM. Results The percentage of the cells at G0/G1 phase increased from 54% in routine culture to 72% in the serum-free RPMI-1640 culture. TGFα treatment of the cells synchronized at G0/G1 phase induced significant increment of cyclin E and D1 expressions (P<0.001), and at the dose of TGFα of 50 μg/L, their expressions increased by 25.18% and 27.52%, respectively (P<0.001). Conclusion TGFα can increase the expression of cyclin E and D1 in gastric carcinoma cells to promote their cell cycle progress.

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