南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1251-1253.

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神经生长因子在胰腺癌中的表达与癌细胞嗜神经侵袭和疼痛的关系

花柱明； 李真； 罗东兰；   

  1. 广东省人民医院麻醉科； 广东省人民医院麻醉科 广东广州510010； 广东广州510010；
  • 出版日期:2006-08-20 发布日期:2006-08-20
  • 基金资助:
    广东省医学科学技术研究基金~~

Relation of nerve growth factor expression with perineural invasion and pain in pancreatic cancer

HUA Zhu-ming, LI Zhen, LOU Dong-lan Department of Anesthesia, Guangdong Provincial People’s Hospital, Guangzhou 510010, China   

  1. 广东省人民医院麻醉科； 广东省人民医院麻醉科 广东广州510010； 广东广州510010；
  • Online:2006-08-20 Published:2006-08-20

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摘要： 目的探讨神经生长因子(NGF)与胰腺癌嗜神经侵袭情况及疼痛症状的关系。方法选择胰腺癌病例28例和正常胰腺组织20例,用Northernblotting和免疫组化的方法,半定量检测NGF表达强度,比较胰腺癌组织与正常胰腺组织的NGF表达强度。对胰腺癌病例进行神经侵袭程度评分和疼痛评分,并与NGF表达强度进行相关分析。结果与对照组相比,胰腺癌标本的NGFmRNA表达水平升高3.1倍(P<0.05)。胰腺癌伴有外周神经侵犯者比无外周神经侵犯者的NGF水平高(P<0.01),肿瘤伴有疼痛者比不伴有疼痛者的NGF水平高(P<0.01)。免疫组化的结果与上述相似。外周神经侵犯的程度与NGF(r=0.66,P<0.01)表达水平呈正相关。疼痛程度与NGF(r=0.63,P<0.01)水平亦呈正相关。外周神经侵犯的程度与NGF(r=0.66,P<0.01)免疫组织化学评分呈正相关。疼痛程度与NGF(r=0.67,P<0.01)免疫组化评分亦呈正相关。结论胰腺癌患者的疼痛发生和胰腺癌的嗜神经性与胰腺癌细胞的NGF表达有关,而与肿瘤的分期、分级无关。 

Abstract: Objective To investigate the association of nerve growth factor (NGF) expression with perineural invasion and pain in pancreatic cancer. Methods NGF expression was detected by Northern blotting and immunohistochemistry in 28 pancreatic cancer and 20 normal pancreatic tissue samples. Correlation analysis of the results with the extent of perineural invasion, pain and histopathologic characteristics of the tumor was performed. Results Northern blot analysis reveled that NGF levels in pancreatic cancer tissues increased by 3.1 folds in comparison with normal pancreas tissue (P<0.05), and immunohistochemistry detected the presence of obvious NGF expression in the cytoplasm of pancreatic cancer cells. Tumors with high NGF expression were associated with more frequent perineural invasion (P<0.01), and increased NGF expression was related to more intense pain (P<0.01). Conclusion Increased NGF expression may contribute to perineural invasion and pain in pancreatic cancer. 

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