南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1234-1236.

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三叶因子在正常胃黏膜中存在的分子形式及其与癌变的关系

任建林； 罗金燕； 卢雅丕； 王琳； 陈建民； 刘明； 施华秀；   

  1. 西安交通大学第二医院消化内科； 厦门大学附属中山医院 厦门市消化病诊治中心； 厦门大学附属中山医院； 厦门市消化病诊治中心； 陕西西安710004厦门大学附属中山医院； 福建厦门361004； 陕西西安710004；
  • 出版日期:2006-08-20 发布日期:2006-08-20
  • 基金资助:
    福建省卫生厅青年科研基金资助(2004-1-26)~~

Molecular forms of TFF1 in normal gastric mucosa and its relationship with canceration

REN Jian-lin1,2, LUO Jin-yan1, LU Ya-pi2, WANG Lin2, CHEN Jian-min2, LIU Ming2, SHI Hua-xiu2 1Department of Gastroenterology, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, China; 2Department of Gastroenterology, Zhongshan Hospital, Xiamen University, Xiamen 361004, China   

  1. 西安交通大学第二医院消化内科； 厦门大学附属中山医院 厦门市消化病诊治中心； 厦门大学附属中山医院； 厦门市消化病诊治中心； 陕西西安710004厦门大学附属中山医院； 福建厦门361004； 陕西西安710004；
  • Online:2006-08-20 Published:2006-08-20

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摘要： 目的研究三叶因子1(TFF1)在正常胃黏膜中存在的分子形式及其在正常、胃癌、不典型增生及肠化胃黏膜中的表达,探讨TFF1在胃癌的发生、发展中的作用及其胃黏膜保护作用的分子生物学机制。方法采用Westernblotting方法观察TFF1在正常胃黏膜中存在的分子形式,并采用免疫组化方法测定正常、胃癌、不典型增生及肠化胃黏膜中TFF1的表达情况,通过图像分析软件分析其阳性信号平均光密度值了解其表达情况。结果TFF1在人正常胃黏膜中以3种形式存在,即单聚体、二聚体和Mr21000的TFF1与某种分子结合而成的复合物,其中TFF1复合物的含量最高。TFF1主要在人胃体及胃窦黏膜上皮表面细胞胞质中表达,核周聚集较明显,越靠近腔面的细胞表达越强。胃腺癌患者癌旁组织TFF1表达(0.51±0.07)明显高于正常胃黏膜(0.44±0.06,P<0.001),而腺癌组织的表达强度则与癌组织的分化程度呈正比,分化程度愈低,表达愈弱,低分化腺癌无阳性表达,中、高分化腺癌表达(0.41±0.07)略低于正常黏膜(P>0.05)。不典型增生胃黏膜TFF1表达(0.57±0.03)明显高于正常胃黏膜(P<0.001);肠化胃黏膜无TFF1阳性表达,但肠上皮化生周围的胃黏膜TFF1表达(0.45±0.07)与正常胃黏膜相比无统计学差异(P>0.05)。结论TFF1在胃黏膜保护及修护方面具有重要的作用,其在胃黏膜中主要以复合物的形式存在。TFF1可能与肿瘤抑制及分化机制有关。

Abstract: Objective To study the molecular forms of TFF1 in normal gastric mucosa and its expression in normal, gastric carcinoma, atypical hyperplasia, and intestinalized gastric mucosa. Methods The molecular forms of TFF1 in normal gastric mucosa was observed by western blotting. The expression of TFF1 in normal, gastric carcinoma, atypical hyperplasia, and intestinalization gastric mucosa was assayed immunohistochemically. Results TFF1 existed in normal gastric mucosa in forms of monomer, dimer and 21-kD TFF1 complex, with the last being the richest. TFF1 was expressed mainly in the epithelial cytoplasm of the mucosa in the gastric body and antrum, especially around the nucleus, and the closer to the lumen, the higher the expression. TFF1 expression in the tissues adjacent to gastric carcinoma was higher than that in normal gastric mucosa (P<0.001), and the expression in gastric adenocarcinoma was positively correlated to differentiation of adenocarcinoma. No TFF1 was expressed in poorly differentiated adenocarcinoma. The expression of TFF1 in moderate and well differentiated adenocarcinoma was a little lower than that in normal mucosa (P>0.05). The gastric mucosa with atypical hyperplasia had significantly higher TFF1 expression than normal gastric mucosa (P<0.001), and TFF1 was not detected in intestinalized gastric mucosa. There was no significant difference in TFF1 expression between gastric mucosa around the intestinalized tissues and normal gastric mucosa (P>0.05). Conclusions TFF1 plays an important part in protection and restitution of the gastric mucosa, and TFF1 may be related to suppression and differentiation of carcinoma.

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