南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1180-1183.

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前列腺素E1对肝缺血/再灌注损伤大鼠Kupffer细胞中单核细胞趋化蛋白-1表达的影响

马玮； 王作仁； 张云峰； 石磊；   

  1. 西安交通大学第一医院肝胆外科； 西安交通大学第一医院肝胆外科 陕西西安710061； 陕西西安710061；
  • 出版日期:2006-08-20 发布日期:2006-08-20

Effect of prostaglandin E1 on monocyte chemotactic protein-1 expression in Kupffer cells of rats with hepatic ischemia-reperfusion injury

MA Wei, WANG Zuo-ren, ZHANG Yun-feng, SHI Lei Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, China   

  1. 西安交通大学第一医院肝胆外科； 西安交通大学第一医院肝胆外科 陕西西安710061； 陕西西安710061；
  • Online:2006-08-20 Published:2006-08-20

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摘要： 目的探讨大鼠肝缺血/再灌注损伤中Kupffer细胞(KCs)中单核细胞趋化蛋白-1(MCP-1)的表达及前列腺素E1(PGE1)对它们的影响。方法72只雄性SD大鼠随机分为假手术组、缺血/再灌注组和PGE1治疗组。建立大鼠常温下部分肝缺血/再灌注损伤模型,PGE1治疗组制模前10min静脉注射PGE1,于1、6、12和24h取下腔静脉血测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平,同时分离KCs,采用ELISA法测定KCs培养上清液中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)的含量,用免疫组化和RT-PCR测定KCs中MCP-1蛋白与mRNA的表达。结果PGE1组血清中ALT和AST明显低于缺血/再灌注组(P<0.05),KCs培养上清液中TNF-α和IL-1β水平及KCs中MCP-1蛋白和mRNA表达较假手术组明显升高(P<0.05),术前应用PGE1可以明显降低这些炎性因子的表达(P<0.05)。结论PGE1对大鼠肝缺血/再灌注损伤具有明显保护作用,可以减少KCs产生的细胞因子TNF-α和IL-1β,降低KCs中MCP-1的表达。 

Abstract: Objective To observe the effect of prostaglandin E1 (PGE1) on the expression of monocyte chemotactic protein-1 (MCP-1) in Kupffer cells (KCs) of rats with hepatic ischemia-reperfusion injury (IRI). Methods Seventy-two SD rats were randomized into sham operation group, ischemia-reperfusion group (I/R group) and PGE1 treatment group (PGE1 group). Rat models of partial warm ischemia-reperfusion injury of the liver was established, and in PGE1 group, PGE1 were given 10 min before the operation. At 1, 6, 12 and 24 h after the reperfusion, blood sample was taken from the inferior vena cava for measuring alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. The KCs were isolated and incubated in vitro, and tumor necrosis factor alpha (TNF-α) and interleukin-1beta (IL-1β) in the supernatant were measured by enzyme-linked immunosorbent assay. MCP-1 expression in the KCs was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Results ALT and AST levels of the PGE1 group were significantly lower than I/R group (P<0.01). The mRNA and protein expression of MCP-1 and the TNF-α and IL-1β levels in the I/R group significantly increased in the course of reperfusion and slightly decreased at 24 h, but were still significantly higher than those in the sham operation group (P<0.05). The expression of these factors were markedly decreased after PGE1 treatment as compared with the I/R group (P<0.05). Conclusion PGE1 can protect against ischemia-reperfusion injury of the rat liver partially by suppressing KCs activation, reducing excessive release of TNF-α and IL-1β from KCs and decreasing the high expression of MCP-1 protein and mRNA. 

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