南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1154-1156.

• • 上一篇    下一篇

肺表面活性物质相关蛋白B在人胎肺发育中的表达及意义

孔祥永； 杜江； 封志纯；   

  1. 南方医科大学珠江医院儿科； 南方医科大学珠江医院儿科 广东广州510282； 广东广州510282；
  • 出版日期:2006-08-20 发布日期:2006-08-20
  • 基金资助:
    广东省科技攻关重点课题(B30502)~~

Surfactant protein B expression and its role in the development of human fetal lung epithelial cells

KONG Xiang-yong, DU Jiang, FENG Zhi-chun Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China   

  1. 南方医科大学珠江医院儿科； 南方医科大学珠江医院儿科 广东广州510282； 广东广州510282；
  • Online:2006-08-20 Published:2006-08-20

PDF

1043

摘要： 目的检测肺表面活性物质相关蛋白B(SP-B)在人胎肺发育过程中的表达特征,探讨其在胎肺上皮细胞发育、分化及其肺功能建立中的作用。方法由孕妇自愿捐献的胎龄为10周￣34周胎肺组织37例及生后28d的内正常肺组织2例,用免疫组织化学技术检测SP-B的表达。结果SP-B在胎肺发育第16周已开始表达,定位于上皮细胞胞浆,发育早期主要表达于高柱状的未分化的上皮细胞,随着支气管的发育分化,由呼吸道的近端逐渐向远端迁移;到原始肺泡期稳定表达于AECⅡ及其前体细胞,其反应强度增加不明显,出生后SP-B阳性反应较出生前明显增强;纤毛细胞和AECI无表达。结论在胎肺发育的过程中,SP-B的分泌反映着肺泡上皮细胞发育成熟,与适应出生后执行功能的需要密切相关。 

Abstract: Objective To investigate the expression patterns of surfactant protein B (SP-B) and its role in the development of human fatal lung epithelial cells. Methods Human fetal lung tissues were obtained from 37 fetuses of 10-34 weeks at abortion with parental consent and from two newborn infants who died of non-pulmonary causes. SP-B expression in the lung tissues was examined by immunohistochemistry. Results SP-B was detected in the cytoplasm of nonciliated columnar epithelial cells of the human fetal lung in as early as the 16th week of gestation. The positive reaction of SP-B was enhanced during canalicular stages and was more intense in the distal than in the proximal airway epithelium. From the 25th week to the prenatal stage, SP-B expression underwent no significant changes in the primitive alveolar stage, but increased remarkably after birth. Conclusion The expression and secretion of SP-B reflects the maturation of the epithelial cells in human fatal lungs, and may closely associate with the survival ability of the newborn infants. 更多

中图分类号: