南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1149-1151.

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不同温度微波消融肝癌效果的酶组织化学检测

刘大全； 吕明德； 谭进富； 王竹； 周忠信；   

  1. 中山大学附属第一医院肝胆外科； 中山大学附属第一医院肝胆外科 广东广州510080； 广东广州510080；
  • 出版日期:2006-08-20 发布日期:2006-08-20
  • 基金资助:
    国家自然科学基金(30471686)~~

Microwave coagulation at different temperatures for hepatocellular carcinoma management: efficacy evaluation by enzyme histochemical staining

LIU Da-quan, LU Ming-de, TAN Jin-fu, WANG Zhu, ZHOU Zhong-xin Department of Hepatobiliary Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China   

  1. 中山大学附属第一医院肝胆外科； 中山大学附属第一医院肝胆外科 广东广州510080； 广东广州510080；
  • Online:2006-08-20 Published:2006-08-20

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摘要： 目的比较HE染色和酶组织化学染色对判断微波消融灭活肝癌细胞效果的价值。方法分别用60℃、3min和50℃、3min两种条件的微波消融,依次治疗A、B两组(每组6只)小鼠移植型肝癌,取微波消融前、后的肿瘤组织标本制成石蜡和冰冻切片,进行常规HE染色及烟酰胺腺嘌呤二核苷酸黄递酶(NADH-diaphorase)化学染色,并在显微镜下观察两种染色情况。结果从HE染色观察,两组小鼠肝癌组织在微波消融后的即刻,其细胞核形态和排列较消融前无明显改变;酶组织化学染色显示,A组肿瘤消融区内的NADH-diaphorase活性均消失,说明癌细胞灭活;B组肿瘤消融区内上述酶活性都呈散在阳性,提示部分癌细胞仍存活;两组肿瘤灭活效果明显不同(P<0.01)。结论HE染色不能评价微波消融对肝癌的即刻灭活效应,酶组织化学染色测定NADH-diaphorase活性能判定微波消融对肝癌的灭活效果。 

Abstract: Objective To compare the application of HE and enzyme histochemical staining in assessing the viability of hepatocellular carcinoma (HCC) cells coagulated by microwave ablation at different temperatures. Methods Two groups of mice (n=6) with transplanted homogenic HCC were treated by microwave ablation at 60 ℃ and 50 ℃ for 3 min, respectively. Before and after microwave ablation, paraffin sections and frozen sections of the tumors were prepared for routine HE staining and enzyme histochemical staining with nicotinamide adenine dinucleotide diaphorase (NADH-diaphorase), respectively, and observed under microscope. Results Shortly after microwave ablation, the morphology and arrangements of the nucleus of the ablated tumor cells in the two groups showed no obvious alteration in HE stained sections, but in sections with enzyme histochemical staining, the activity of NADH-diaphorase in ablated tumor tissue at 60 ℃ disappeared, suggesting the death of HCC cells; sporadic activity of the enzyme was detected in the coagulated tumor at 50 ℃, indicating tumor cells surviving the ablation. The ablation effect was markedly different between the two groups (P<0.01). Conclusion HE staining is not suitable for evaluation of HCC destruction immediately after microwave ablation, and detection of NADH-diaphorase activity with the enzyme histochemical method better suits this purpose. 

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