南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1136-1139.

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Luminex液相芯片技术测定人早孕滋养层细胞及蜕膜细胞中基质金属蛋白酶的表达

贺晓恒； 陈士岭； 孙玲； 邢福祺；   

  1. 南方医科大学南方医院生殖医学中心； 南方医科大学南方医院生殖医学中心 广东广州510515； 广东广州510515；
  • 出版日期:2006-08-20 发布日期:2006-08-20
  • 基金资助:
    国家自然科学基金(30470657);广东省自然科学基金(04020416)~~

Expression of matrix metalloproteinases in the cytotrophoblasts and decidual stromal cells in human early pregnancy

HE Xiao-heng, CHEN Shi-ling, SUN Ling, XING Fu-qi Center for Reproductive Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学南方医院生殖医学中心； 南方医科大学南方医院生殖医学中心 广东广州510515； 广东广州510515；
  • Online:2006-08-20 Published:2006-08-20

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摘要： 目的研究人正常早孕滋养层细胞与蜕膜细胞中基质金属蛋白酶(MMPs)的表达特点,探讨子宫内膜蜕膜化进程中MMPs的变化及对胚胎着床的影响。方法收集正常妇女分泌期子宫内膜及人正常早孕绒毛和相应蜕膜标本各5例,分别进行离体培养,鉴定其纯度在98%以上后,收集培养上清液,利用Luminex(LumAvidinBeads)测定培养上清液中MMPs(MMP-1、MMP-2、MMP-3、MMP-7、MMP-8、MMP-9、MMP-12、MMP-13)蛋白表达的变化,分析MMPs在不同培养条件下的表达差异及表达之间的相关性。结果①在不同培养条件下,MMP-1、MMP-2、MMP-3、MMP-7、MMP-8、MMP-9均有表达,MMP-12及MMP-13分别在ESC及DSC中无表达,且MMP-8、MMP-12、MMP-13表达均低。②与子宫内膜细胞相比,MMP-1、MMP-3、MMP-7在DSC,EVCT中表达均降低,降低有显著性差异(P<0.05),而MMP-2、MMP-8、MMP-9在DSC,EVCT中则显著升高,升高有显著性差异(P<0.05)。且在蜕膜细胞中,MMP-1、MMP-3、MMP-2、MMP-7高表达尤为显著。③与蜕膜细胞相比,MMP-1、MMP-3、MMP-7在EVCT中表达显著降低(P<0.05)。MMP-2、MMP-9、MMP-13在EVCT中升高有显著性(P<0.05)。且在滋养层细胞中,MMP-2、-9高表达尤为显著。④MMPs之间的相关性:MMP-2与MMP-9相关关系显著(P<0.05),且关系较密切(r=0.6645)。MMP-1,MMP-3,MMP-7两两间相关关系显著(P<0.05),且关系密切(r>0.7340)。其他MMP之间相关关系不显著。结论首次利用LuminexxMAP方法多通量的检测到子宫内膜间质细胞、早孕蜕膜细胞与滋养层细胞的多种MMPs的表达,它们均可分泌多种MMP,但是不同种类组织细胞的表达量不同;MMPs可能通过调控子宫内膜蜕膜化及母胎界面间MMPs的变化而影响胚胎着床,为成功妊娠创造条件。

Abstract: Objective To study the expression of matrix metalloproteinases (MMPs ) in the decidual stromal cells (DSCs) and extravillous cytotrophoblasts (EVCT) in human early pregnancy and explore the change of MMPs in endometrial stromal cell (ESC) decidualization and its impact on implantation and placentation. Methods The decidua and villi from 5 women with early pregnancy and mid-secretory endometrium from 5 normal women were collected and cultured in vitro, and the supernatants of the culture media were collected after 48 hours of incubation. The expression of the MMPs in the ESCs, DSCs and EVCTs was detected using Luminex xMAP system simultaneously and the difference in MMPs expression and their correlations were analyzed with SPSS10.0 software. Results The MMPs (MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, and MMP-9) were expressed in ESCs, DSCs and EVCTs, while MMP-12 was not found in ESCs and MMP-13 not in DSCs. Expressions of MMP-8, MMP-12, and MMP-13 were lowered. Compared with the ESCs, DSCs and EVCTs showed significantly lowered expressions of MMP-1, MMP-3, and MMP-7 (P<0.05), whereas expression of MMP-2 and MMP-9 increased significantly, and the high expressions of MMP-1, MMP-3, and MMP-7 was especially obvious in the DSCs. The expressions of MMP-1, MMP-3, and MMP-7, however, were significantly decreased in the EVCTs in comparison with the DSCs. Significant correlations were noted between MMP-1, MMP-3, and MMP-7, and MMP-2 was closely correlated with MMP-9. MMP-8 was significantly lower and MMP-12 and MMP-13 showed no obvious variation in the cell culture. Conclusion MMPs are secreted by ESCs, DSCs and EVCTs. Diverse MMPs play an important role in proliferation and differentiation of the ESC to affect embryo implantation and placentation. All MMPs establish a balance to co-regulate the process of pregnancy. 

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