南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (08): 1121-1123.

• • 上一篇    下一篇

冷冻保存后大鼠胰岛细胞与小肠黏膜下层的共培养

侯军； 项和立； 冯新顺； 田小辉； 庞新路； 藤焱；   

  1. 西安交通大学医学院第一附属医院肾移植中心； 西安交通大学医学院第一附属医院肾移植中心 陕西西安710061； 陕西西安710061；
  • 出版日期:2006-08-20 发布日期:2006-08-20
  • 基金资助:
    国家自然科学基金(30571799);教育部科学技术重点项目(104169,2004-2007)~~

Co-culture of cryopreserved rat islets with small intestinal submucosa

HOU Jun, XIANG He-li, FENG Xin-shun, TIAN Xiao-hui, PANG Xin-lu, TENG Yan Center of Renal Transplantation, First Hospital of Xi’an Jiaotong University, Xi’an 710061, China   

  1. 西安交通大学医学院第一附属医院肾移植中心； 西安交通大学医学院第一附属医院肾移植中心 陕西西安710061； 陕西西安710061；
  • Online:2006-08-20 Published:2006-08-20

PDF

976

摘要： 目的探讨猪小肠黏膜下层(SIS)对冷冻保存后大鼠胰岛细胞的支持和保护作用。方法分离纯化后的Wistar大鼠胰岛细胞经冷冻保存1月后分为SIS组和对照组,在RPMI1640培养液培养1周后分别测定两组的胰岛细胞回收率,观察胰岛细胞形态并进行胰岛素刺激释放试验。结果SIS组胰岛细胞回收率为(91.7±1.8)%,较游离组(60.6±3.3)%显著提高(P<0.05),胰岛形态较对照组完整。在高糖(16.7mmol/L)刺激下,SIS组胰岛素分泌量较游离组增高[(23.7±1.6)vs(12.5±1.1)mU/L,P<0.05)]。在含有茶碱的高糖溶液中,SIS组的刺激指数为对照组的3倍。结论SIS作为一种天然的细胞外基质材料,可显著提高胰岛细胞冷冻保存后的回收率,减少冻存胰岛细胞复苏时的死亡,并改善胰岛细胞的功能。

Abstract: Objective To investigate the feasibility and benefits of co-culture of cryopreserved islets with small intestinal submucosa (SIS). Methods Purified rat islets cryopreserved for one month were divided into SIS group and control group, and after culture in standard islet culture media RPMI1640 for 1 week, the morphology and function of the islets were assessed. Results The SIS protects the fragile islets from damage by cryopreservation, and increased the recovery from (60.6±3.3)% to (91.7±1.8)%(P<0.05). Compared with the control group, incubation of the islets of the SIS group in high-glucose (16.7 mmol/L) solution resulted in significantly enhanced insulin secretion (23.7±1.6 vs 12.5±1.1 mU/L, P<0.05). When the islets were incubated in high-glucose solution containing theophylline, the calculated stimulation index of SIS group was about 3-fold higher than that of the control group. Conclusion Co-culture of cryopreserved rat islets with SIS can increase the recovery of islet cells and improve their function. 

中图分类号: