南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (07): 931-935.

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磁共振灌注成像监测组织工程骨血管化的实验研究

王学明； 裴国献； 金丹； 魏宽海； 江汕； 唐光辉；   

  1. 南方医科大学南方医院创伤骨科； 南方医科大学南方医院创伤骨科 广东广州510515； 广东广州510515；
  • 出版日期:2006-07-20 发布日期:2006-07-20
  • 基金资助:
    国家高技术发展计划(863计划)重大专项(2003AA205001)

Perfusion-weighted magnetic resonance imaging for monitoring vascularization in tissue-engineered bone in rhesuses

WANG Xue-ming, PEI Guo-xian, JIN Dan, WEI Kuan-hai, JIANG Shan, TANG Guang-hui Department of Traumatology and Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学南方医院创伤骨科； 南方医科大学南方医院创伤骨科 广东广州510515； 广东广州510515；
  • Online:2006-07-20 Published:2006-07-20

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摘要： 目的探讨磁共振灌注成像在组织工程骨血管化监测中的应用价值。方法在13只恒河猴的25个胫骨上制备20mm的骨膜与骨缺损,依据填充的材料不同随机分为五组,A组:β-磷酸三钙(β-TCP)+骨髓基质干细胞(BMSCs)+血管束;B组:β-TCP+血管束;C组:β-TCP+BMSCs;D组:β-TCP;E组:空白对照。术后4、8、12周行磁共振灌注成像检查并计算信号强度-时间(SI-T)曲线的最大线性斜率(SSmax)和基线值(SIbaseline),拍摄恒河猴胫骨X线片并计算其透光度,同时行放射性核素骨显像检查。结果术后4、8、12周A组的SSmax值最高,术后8周与4周相比SSmax有较大幅度的提高(P=0.003)。术后8周A组5个样本的同位素计数比值与磁共振灌注成像检查的SSmax呈正相关关系(rs=0.899,P=0.038),术后12周A组5个样本的SSmax与X线片透光度呈负相关(rs=-0.892,P=0.042)。结论SI-T曲线的SSmax能够准确地反映组织工程骨的血管化情况,磁共振灌注成像检查具有无创、无辐射、高灵敏度和可定量分析的优点。 

Abstract: Objective To assess the value of perfusion-weighted magnetic resonance (MR) imaging (PWMRI) in monitoring vascularization in tissue-engineered bone graft. Methods Tibial diaphyseal defect of 20 mm was induced in 25 lower limbs of 13 rhesuses and fixed with an AO reconstruction plate with 7 holes. The monkeys were randomized into 5 groups according to the materials used for defect filling: group A, with β-tricalcium phosphate (β-TCP), bone marrow stromal cells (BMSCs) and blood vessel bundles; group B, with β-TCP and blood vessel bundles; group C, with β-TCP and BMSCs; group D, with β-TCP, and group E without filling. PWMRI, X-ray, and radionuclide imaging were carried out at weeks 4, 8, 12 postoperatively. The maximum slope rates of the single intensity-time curve (SSmax) and the baseline values (SIbaseline) on the same time points were calculated. Transmittances on the X-ray films and isotope counts in the region of interest (ROI) were assessed and calculated. Results Compared with other groups, group A showed the highest SSmax at weeks 4, 8, and 12 postoperatively, and its SSmax at week 8 was significantly higher than that at week 4 (P=0.003). The SSmax was positively related to isotope counts in ROI at week 8 after operation (rs=0.899, P=0.038), and inversely related to transmittance on X-ray films at week 12 (rs=-0.892, P=0.042). Conclusion The SSmax of the single intensity-time curve can accurately reflect the vascularization of the tissue-engineered bone graft, and PWMRI allows sensitive, quantitative, noninvasive and radiation-free vascularization monitoring. 

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