南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (07): 1053-1054.

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从噬菌体随机十二肽库中筛选乙酰胆碱酯酶的抑制肽

张兴梅； 石玉生； 王春霞；   

  1. 南方医科大学基因工程研究所； 南方医科大学南方医院放疗科； 南方医科大学南方医院药学部 广东广州510515； 广东广州510515；
  • 出版日期:2006-07-20 发布日期:2006-07-20

Screening of peptide inhibitors of acetylcholinesterase from 12-mer random phage display peptide library

ZHANG Xing-mei1, SHI Yu-sheng2, WANG Chun-xia3 1Institute of Genetic Engineering, Southern Medical University, Guangzhou 510515, China; Department of Radiotherapy2, Department of Pharmacy3, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学基因工程研究所； 南方医科大学南方医院放疗科； 南方医科大学南方医院药学部 广东广州510515； 广东广州510515；
  • Online:2006-07-20 Published:2006-07-20

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摘要： 目的从随机肽库中筛选乙酰胆碱酯酶(AChE)的抑制性多肽。方法以人AChE作为靶标,应用噬菌体随机12肽库进行筛选,经过3轮筛选,对阳性克隆进行测序并进行序列分析及抑酶活性测定。结果筛选得到6个能与人AChE较强结合的噬菌体克隆,其中有4个克隆可以抑制AChE的酶活性,其保守序列为W(S/P)HY。结论通过噬菌体肽库技术能够筛选到抑制人AChE活性的多肽,为进一步研究AChE的多肽抑制剂奠定了基础。 

Abstract: Objective To screen the peptide inhibitor of acetylcholinesterase (AChE) from 12-mer random phage display peptide library. Methods Human AChE was used as the target to screen its binding peptides from 12-mer random phage display peptide library. The positive phage clones were isolated after three rounds of biopanning followed then by sequence analysis and their activity evaluation. Results Six positive phage clones binding to human AChE were obtained, and 4 of them sharing the conservative sequence W(S/P)HY inhibited the enzyme activity of AChE. Conclusion Acquisition of AChE inhibitor from phage display library provides clues for designing peptide inhibitors of AChE. 

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