南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (07): 1050-1052.

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人类早孕蜕膜对B淋巴细胞IgG分泌的影响

胡冬梅； 曹咏清； 陈竹钦； 王妮；   

  1. 南方医科大学珠江医院妇产科； 中国科学院动物研究所计划生育生殖生物学国家重点实验室； 第三军医大学大坪医院妇产科； 中国科学院动物研究所计划生育生殖生物学国家重点实验室 广东广州510282； 北京100080； 重庆400042；
  • 出版日期:2006-07-20 发布日期:2006-07-20

Effect of exogenous cytokine-stimulated decidual cells of early pregnancy on IgG secretion of B lymphocytes

HU Dong-mei1, CAO Yong-qing2, CHEN Zhu-qin3, WANG Ni2 1Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China; 2State Key Laboratory of Reproductive Biology, Beijing 100080, China; 3Department of Obstetrics and Gynecology, Daping Hospital, Third Military Medical University, Chongqing 400042, China   

  1. 南方医科大学珠江医院妇产科； 中国科学院动物研究所计划生育生殖生物学国家重点实验室； 第三军医大学大坪医院妇产科； 中国科学院动物研究所计划生育生殖生物学国家重点实验室 广东广州510282； 北京100080； 重庆400042；
  • Online:2006-07-20 Published:2006-07-20

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摘要： 目的观察细胞因子对蜕膜细胞介导B淋巴细胞分泌IgG的影响,探讨蜕膜局部免疫微环境的免疫学特点。方法将γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-6(IL-6)和表皮生长因子(EGF)加入培养的蜕膜细胞中,用蜕膜细胞的培养上清液刺激B淋巴细胞生长,通过放射免疫法测定作用不同时间后B细胞分泌的IgG的值。结果正常早孕蜕膜细胞的培养上清液可以刺激B淋巴细胞IgG的分泌;经细胞因子作用后的蜕膜细胞培养上清液使B细胞分泌的IgG明显上升,但这种作用与细胞因子的浓度、种类无明显相关性,而与细胞因子作用的时间具有一定关联,表现在第12小时实验组的IgG分泌显著上升(P<0.05),到24、48h时实验组有逐渐下降趋势。结论在外源性细胞因子的作用下,蜕膜局部体液免疫反应增强,但这种增强可能是蜕膜局部免疫的自身调节作用,以维护妊娠的正常进行。 

Abstract: Objective To study the effect of exogenous cytokine-stimulated decidual cells on IgG secretion of B lymphocytes and investigate the features of local immunological microenvironment of the decidua. Methods Exogenous cytokines interferon-γ (IFN-γ), interleukin-2 (IL-2), IL-6 and epidermal growth factor (EGF) were added separately in cultured decidual cells, and the supernatant of the culture medium was prepared for stimulating human peripheral blood lymphocytes. IgG secretion of the B cells was measured by radio-immunological method. Result The decidual cells of normal early pregnancy stimulated B lymphocyte IgG secretion, and the supernatant of exogenous cytokine-stimulated decidual cells had the same effect, which, however, was depended not on the concentration and category of the cytokines, but only on the time of treatment. Conclusion The exogenous cytokines can increase the humoral immunit in the decidual immunological microenvironment, but such effect might result from a self-regulatory mechanism of the local immunological microenvironment of the decidua, which can be fundamental for maintenance of normal pregnancy. 

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