南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (06): 780-783.

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灵芝多糖拮抗前列腺素E2对小鼠脾细胞IFN-γ和TNF-α mRNA表达的抑制作用

张群； 雷林生； 朱正光； 余传林； 吴曙光；   

  1. 南方医科大学药学院药物研究所； 南方医科大学药学院药物研究所 广东广州510515； 广东广州510515；
  • 出版日期:2006-06-20 发布日期:2006-06-20
  • 基金资助:
    广东省自然科学基金(04020361)~~

Ganoderma polysaccharides antagonize prostaglandin E2-induced suppression of murine splenocyte IFN-γ and TNF-α mRNA expression

ZHANG Qun, LEI Lin-sheng, ZHU Zheng-guang, YU Chuan-lin, WU Shu-guang Institute of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学药学院药物研究所； 南方医科大学药学院药物研究所 广东广州510515； 广东广州510515；
  • Online:2006-06-20 Published:2006-06-20

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摘要： 目的观察灵芝多糖拮抗前列腺素E2(PGE2)对小鼠脾细胞干扰素γ(IFN-γ)和肿瘤坏死因子α(TNF-α)mRNA表达的抑制作用。方法混合淋巴细胞培养反应作为实验模型,半定量RT-PCR方法检测IFN-γ和TNF-αmRNA的表达水平。结果PGE2连续作用4h后,脾细胞IFN-γmRNA的表达与对照组比较受到不同程度的抑制,当PGE2浓度在10μmol/L以上时具有统计学意义(P<0.01)。固定PGE2浓度为20μmol/L,合用不同浓度的灵芝多糖,当灵芝多糖为100mg/L以上时可部分对抗PGE2的抑制作用。培养8h后,PGE2明显抑制TNF-αmRNA的表达,灵芝多糖为100mg/L以上时可部分对抗。结论灵芝多糖可部分拮抗PGE2对小鼠脾细胞IFN-γ和TNF-αmRNA表达的抑制作用。

Abstract: Objective To determine if Ganoderma polysaccharides can antagonize prostaglandin E2 (PGE2)-induced suppression of murine splenocyte interferon γ (IFN-γ) and tumor necrosis factor α (TNF-α) mRNA expression. Methods Mixed lymphocyte culture reaction was used as the experimental model. The expressions levels of IFN-γ and TNF-α mRNA were measured by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results After the cultures were treated with PGE2 for 4 h, IFN-γ mRNA expression was reduced as compared with the control, which was especially obvious when PGE2 concentrations exceeded 10 μmol/L (P<0.01). Ganoderma polysaccharides above 100 mg/L showed partial antagonistic effect against the inhibition of IFN-γ by PGE2 at the fixed concentration of 20 μmol/L. Further studies indicated that PGE2 (20 μmol/L) impaired the expression of TNF-α mRNA after an 8-hour incubation and Ganoderma polysaccharides above 100 mg/L could partially antagonize this effect. Conclusion Ganoderma polysaccharides can partially antagonize PGE2-induced suppression of murine splenocyte IFN-γ and TNF-α mRNA expression. 

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