南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 664-666.

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膜迷路破坏动物模型中水通道蛋白-1的表达及其意义

李琦； 黄德亮；   

  1. 解放军总医院耳鼻咽喉研究所； 解放军总医院耳鼻咽喉-头颈外科 北京100853； 北京100853；
  • 出版日期:2006-05-20 发布日期:2006-05-20

Expression of aquaporin-1 in a guinea pig model of labyrinth destruction

LI qi1,HUANG De-liang2 1Institute of Otolaryngology,2Department of Otolaryngology-Head and Neck Surgery,General Hospital of PLA,Beijing 100853,China   

  1. 解放军总医院耳鼻咽喉研究所； 解放军总医院耳鼻咽喉-头颈外科 北京100853； 北京100853；
  • Online:2006-05-20 Published:2006-05-20

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摘要： 目的检测水通道蛋白-1在膜迷路破坏豚鼠耳蜗及内淋巴囊中的表达情况。方法以氯仿鼓室注射制造豚鼠膜迷路破坏的动物模型,运用免疫组化二步法在不同的时间点上检测膜迷路破坏豚鼠耳蜗及内淋巴囊中水通道蛋白-1的表达。结果耳蜗中AQP-1的表达表现出一种波动性过程,即随螺旋韧带细胞形态的破坏出现下调,而后当螺旋韧带细胞出现再生时AQP-1的表达出现上调。在内淋巴囊处水通道蛋白-1的表达则无明显改变。结论水通道蛋白-1可能参与维持耳蜗螺旋韧带处结构的稳定性。

Abstract: Objective To study the expression of aquaporin-1(AQP-1)in the cochlea and endolymphatic sac in guinea pigs with labyrinth destruction.Methods Chloroform was injected into the tympanum to establish the animal model of labyrinth destruction in guinea pigs,and two-step immunohistochemical method was used to examine the expression of AQP-1 in the cochlea and endolymphatic sac at different time points.Results AQP-1 expression was fluctuant in accordance with the morphological changes of the spiral ligament fibrocytes in the cochlea:destruction of the spiral ligament cells was followed by AQP-1 expression down-regulation,and AQP-1 up-regulation occurred with the cell regeneration.But no such changes were observed in the endolymphatic sac.Conclusion AQP-1 may take part in the maintenance of the structural stability of the spiral ligament. 

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