南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 635-637.

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医患鼻前庭携带耐甲氧西林金黄色葡萄球菌与医院感染菌同源性的研究

张亚莉; 周宏伟; 马骊; 温茜; 王茵茵; 李琼; 汪能平;   

  1. 南方医科大学南方医院医院感染管理科; 香港城市大学生物与化学系; 南方医科大学生物技术学院分子免疫学研究所; 南方医科大学南方医院医院感染管理科 广东广州510515; 广东广州510515;
  • 出版日期:2006-05-20 发布日期:2006-05-20
  • 基金资助:
    2004年南方医院院长基金

Homology analysis of the epidemiological strains of meticillin-resistant Staphylococcus aureus and the strains isolated from the nasal fossa of the medical staff and inpatients

ZHANG Ya-li1,ZHOU Hong-wei2,MA Li3,WEN Qian3,WANG Yin-yin1,LI Qiong1,WANG Neng-ping1 1Department of Infection Control,Nanfang Hospital,3Institute of Molecular Immunology,Southern Medical University,Guangzhou 510515,China;2City University of Hong Kong,Hong Kong,China   

  1. 南方医科大学南方医院医院感染管理科; 香港城市大学生物与化学系; 南方医科大学生物技术学院分子免疫学研究所; 南方医科大学南方医院医院感染管理科 广东广州510515; 广东广州510515;
  • Online:2006-05-20 Published:2006-05-20

摘要: 目的探讨医院医务人员和住院患者鼻前庭带耐甲氧西林金黄色葡萄球菌(MRSA)的相互关系和对发生与控制医院感染的影响。方法对本院发生金葡菌感染较多的某科医务人员和住院1周以上患者的鼻前庭进行采样,常规方法进行金葡菌鉴定和MRSA确认。将医患鼻前庭以及肺炎患者痰中分离出的MRSA菌株,进行随机引物扩增多态DNA(RAPD)检测,将RAPD指纹图输入凝胶成像分析系统,经计算机处理,运用RAPD软件(Quantityone4.4.0Bio-Rad)进行同源性分析。判断医患鼻前庭携带MRSA的相关性及其与MRSA医院感染的相关性。结果RAPD聚类分析,基因同源性较高的可分为5型,医患之间、患者之间鼻前庭以及3株MRSA院内肺炎与其本人鼻前庭所携带的MRSA有较高的同源性。结论MRSA在鼻前庭较的定植或携带,可通过患者、医务人员的移动发生病室内、病室间的患者之间和医患之间的传播或感染。 

Abstract: Objective To investigate the relation between the epidemiological strains of meticillin-resistant Staphylococcus aureus(MRSA)and the strains isolated from the nasal fossa of the medical staff and inpatients.Methods The MRSA strains were isolated from the nasal fossa of the medical staff and inpatients in the Department of Neurosurgery.The genes of the isolated strains were amplified by randomly amplified polymorphic DNA(RAPD)assay.Results Three and 12 MRSA strains were isolated from the nasal fossa of the medical staff and patients who were hospitalized for more than 1 week,respectively,and RAPD assay revealed high homology between the isolated strains.Conclusion Cross infection can be present between the medical staff,inpatients,and the infected patients. 

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