南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 617-619.

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抗原致敏树突状细胞诱导CTL杀伤K562细胞体外实验研究

华佳叶； 黄仁魏；   

  1. 广州医学院第二附属医院血液科； 中山大学附属第三医院血液科 广东广州510260中山大学附属第三医院血液科； 广东广州510630；
  • 出版日期:2006-05-20 发布日期:2006-05-20
  • 基金资助:
    广东省自然科学基金(31713)~~

In vitro study of cytotoxic T lymphocyte activation by antigen-loaded dendritic cells for killing of K562 cells

HUA Jia-ye1,HUANG Ren-wei2 1Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China;2Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China   

  1. 广州医学院第二附属医院血液科； 中山大学附属第三医院血液科 广东广州510260中山大学附属第三医院血液科； 广东广州510630；
  • Online:2006-05-20 Published:2006-05-20

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摘要： 目的研究体外负载K562细胞冻融抗原的树突状细胞(DC)诱导细胞毒性T淋巴细胞(CTL)的特异性杀伤作用。方法联合应用细胞因子从外周血单个核细胞(PBMC)诱导培养出DC,在体外负载K562细胞冻融抗原。采用乳酸脱氢酶释放法,对比K562冻融抗原致敏DC组、未致敏DC组、淋巴细胞组、前列腺癌PC3冻融抗原致敏DC组分别激活的CTL对K562细胞的杀伤作用。结果培养出具有典型特征的DC;在效靶比为10:1及20:1时,K562冻融抗原致敏DC组诱导的CTL对K562细胞的杀伤率分别为55.9%±22.0%、90.2%±24.8%,均高于其他3组(P<0.05)。结论K562细胞冻融抗原致敏DC可诱导激活CTL,具有明显杀伤K562细胞作用,并具有特异性。 

Abstract: Objective To study the effect of dendritic cells(DC)stimulated with K562 cell lysate in inducing specific cytotoxic T lymphocytes(CTL)against K562 cells in vitro.Methods The DCs were derived from healthy human peripheral blood monocytes in the presence of granulocyte-macrophage colony-stimulating factor,interleukin(IL)-4 and tumor necrosis factor(TNF)α.The T cells were stimulated by DCs loaded with freeze-thawed K562 cells and T-cell cytotoxicities were measured by lactate dehydrogenase(LDH)assay.Results The DCs could be successfully obtained from peripheral blood monocyte after the culture.Mixed lymphocyte reactions induced by the antigen-loaded DC were much stronger than those induced by human peripheral blood monocytes(P<0.05).At the effector to target ratio of 10:1 and 20∶1,cytotoxicities against K562 cells by CTL derived from culture with the antigen-loaded DCs were the strongest(P<0.05).Conclusion CTL derived from DCs pulsed with K562 cell lysate show effecitive and specific cytotoxicity against K562 cells. 

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