南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 615-616.

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油酸肺损伤时肺泡II型细胞钠转运功能的变化

李涛平； 申海燕；   

  1. 南方医科大学南方医院呼吸科； 南方医科大学南方医院呼吸科 广东广州510515； 广东广州510515；
  • 出版日期:2006-05-20 发布日期:2006-05-20
  • 基金资助:
    国家自然科学基金;广东省自然科学基金~~

Sodium transport of alveolar type Ⅱ cells in oleic acid-induced acute respiratory distress syndrome

LI Tao-ping,SHEN Hai-yan Department of Respiratory Diseases,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学南方医院呼吸科； 南方医科大学南方医院呼吸科 广东广州510515； 广东广州510515；
  • Online:2006-05-20 Published:2006-05-20

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摘要： 目的观察油酸肺损伤模型中肺泡Ⅱ型上皮细胞钠主动转运功能的改变。方法建立大鼠油酸急性呼吸窘迫综合征(ARDS)模型后,分离纯化肺泡Ⅱ型上皮细胞,利用膜片钳全细胞记录模式研究钠转运功能状态以及β2受体激动剂特布他林对其的影响。结果ARDS大鼠肺泡Ⅱ型上皮细胞钠主动转运功能明显减弱,但是特布他林可以使全细胞钠转运增加。结论ARDS时肺泡上皮细胞钠主动转运功能仍然存在,但较正常时明显减弱;而特布他林仍可增加上皮钠转运。

Abstract: Objective To observe the changes of sodium transport of alveolar type Ⅱ cells in oleic acid-induced acute respiratory distress syndrome(ARDS).Methods After isolation of the ATⅡcells as ARDS model induced by oleic acid,the whole cell sodium current was recorded in 5 h with patch-clamp in whole-cell mode.The effect of β2-agonist terbutaline on the current was also examined.Results The sodium currents recorded from ATⅡcells in ARDS were smaller than that of the control cells,but the current could be stimulated to increase obviously by terbutaline.Conclusion ATⅡcells can actively transport sodium ion even under conditions of ARDS,but this capacity can not match that of the control cells.Terbutaline can obviously stimulate the sodium current. 

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