南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 570-572.

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载体介导HER-2RNA干扰治疗乳腺癌研究

胡孝渠； 秦利； 苏逢锡； 姚和瑞； 陈积圣； 龚畅； 郭巨江； 于风燕； 贾海霞；   

  1. 中山大学第二医院乳腺肿瘤中心； 中山大学第二医院肿瘤内科； 中山大学第二医院乳腺肿瘤中心 广东广州510272； 广东广州510272；
  • 出版日期:2006-05-20 发布日期:2006-05-20
  • 基金资助:
    国家自然科学基金(30440021);国家杰出青年自然科学基金(30525022)~~

Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer

HU Xiao-qu1,QIN Li1,SU Feng-xi1,YAO He-rui2,CHEN Ji-sheng1,GONG Chang1,GUO Ju-jiang1,YU Feng-yan1,JIA Hai-xia1 1Center of Breast Cancer,2Department of Oncology,Second Hospital of Sun Yat-sen University,Guangzhou 510272,China   

  1. 中山大学第二医院乳腺肿瘤中心； 中山大学第二医院肿瘤内科； 中山大学第二医院乳腺肿瘤中心 广东广州510272； 广东广州510272；
  • Online:2006-05-20 Published:2006-05-20

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摘要： 目的探讨载体介导HER-2RNA干扰治疗HER-2阳性乳腺癌的可行性。方法构建能够产生HER-2siRNA的质粒载体,利用载体转染HER-2阳性乳腺癌细胞SKBR-3,利用RT-PCR与Westernblot检测HER-2mRNA与蛋白表达情况,同时观察转染后SKBR-3细胞凋亡及生长情况。结果SKBR-3细胞HER-2受到载体介导的RNA干扰后,HER-2mRNA及蛋白表达出现明显下调,细胞凋亡增加,肿瘤细胞生长受到抑制。结论乳腺癌HER-2是理想的RNA干扰治疗靶点,靶向HER-2的RNA干扰可以诱导肿瘤凋亡,抑制肿瘤生长。

Abstract: Objective To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.Methods A plasmid vector capable of mediating HER-2 RNA interference was constructed,and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector.The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting,and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.Results The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference,which also resulted in tumor cell growth inhibition and increased number apoptotic cells.Conclusion HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.

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