南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 567-569.

• • 上一篇    下一篇

小鼠c-Kit~+lin-骨髓细胞移植生成肝细胞的实验研究

唐浩； 廖彩仙； 周杰； 金浩生； 谭远飞； 苏俊； 张春兴； 张守华；   

  1. 南方医科大学南方医院肝胆外科； 南方医科大学南方医院肝胆外科 广东广州510515； 广东广州510515；
  • 出版日期:2006-05-20 发布日期:2006-05-20
  • 基金资助:
    国家自然科学基金(30371385);广东省自然科学基金(04020419)~~

Differentiation of transplanted mouse c-Kit+lin-bone marrow cells into hepatocytes in vitro

TANG Hao,LIAO Cai-xian,ZHOU Jie,JIN Hao-sheng,TAN Yuan-fei,SU Jun,ZHANG Chun-xing,ZHANG Shou-hua Department of Hepatobiliary Surgery,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学南方医院肝胆外科； 南方医科大学南方医院肝胆外科 广东广州510515； 广东广州510515；
  • Online:2006-05-20 Published:2006-05-20

PDF

1217

摘要： 目的评价小鼠c-Kit+Lin-骨髓细胞的肝干细胞潜能,为骨髓源性肝干细胞的临床运用提供可行性实验依据。方法供体为纯系BALB/C雄性小鼠,c-Kit+Lin-骨髓细胞采用免疫磁珠分选法分离细胞。受体为行35Gy全肝照射处理后的同龄同系BALB/C雌性小鼠,立即移植供体c-Kit+lin-骨髓细胞。接受c-Kit+lin-骨髓细胞移植1月后,活杀取肝做常规病理学检查、Y染色体的原位杂交检查、甲胎蛋白与白蛋白的免疫组化检测。结果移植1月后小鼠肝脏的组织结构已恢复正常,肝组织细胞中存在原位杂交和免疫组化均呈阳性反应的双阳性细胞。结论小鼠c-Kit+Lin-骨髓细胞具有肝干细胞潜能,移植后可以在肝内定居并分化形成肝细胞。可做为肝病细胞移植疗法的种子细胞。 

Abstract: Objective To ascertain whether mouse c-Kit+Lin-bone marrow cells have the potential of hepatic stem cells.Methods c-Kit+lin-bone marrow cells were isolated and purified by magnetic-activated cell sorting(MACS)from BALB/C male donor mice,and immediately transplanted into age-matched BALB/C syngeneic female mice with 35-Gy total liver irradiation.The recipients were sacrificed 1 month after the transplantation for pathological observation of the liver morphology.The presence of Y-chromosome was examined in the liver cells of the recipient by in situ hybridization(ISH),and alpha-fetoprotein(AFP)and albumin in the cells were detected by immunohistochemistry.Results The hepatocytes positive for Sry gene on Y-chromosome were identified 1 month after transplantation,and immunohistochemistry for AFP and albumin confirmed that the donor mice-derived cells were hepatocytes.Conclusion c-Kit+lin-bone marrow cells have the potential of hepatic stem cells,which can reside and differentiate into hepatocytes in the liver after transplantaion.c-Kit+lin-bone marrow cells can be used as the source cells of cell transplantation for liver disease.

中图分类号: