南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (05): 561-563.

• • 上一篇    下一篇

兔自体骨髓干细胞移植对缺血再灌注损伤肾功能的影响

肖丽佳； 李亚洁； 余月明； 潘兴华； 曹礼应； 杨勇琴；   

  1. 南方医科大学南方医院护理部； 成都军区昆明总医院肾脏科； 成都军区昆明总医院中心实验科； 成都军区昆明总医院中心实验科 广东广州510515； 广东广州510515； 云南昆明650032；
  • 出版日期:2006-05-20 发布日期:2006-05-20
  • 基金资助:
    云南省自然科学基金(2003C0013Z)~~

Effect of autologous bone marrow stem cell transplantation on renal function following renal ischemic-reperfusion in rabbits

XIAO Li-jia1,LI Ya-jie1,YU Yue-ming2,PAN Xing-hua3,CAO Li-ying2,YANG Yong-qin3 1Department of Nursing,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;2Department of Nephrology,3Central Laboratory,Kunming General Hospital of Chengdu Command,Kunming 650032,China   

  1. 南方医科大学南方医院护理部； 成都军区昆明总医院肾脏科； 成都军区昆明总医院中心实验科； 成都军区昆明总医院中心实验科 广东广州510515； 广东广州510515； 云南昆明650032；
  • Online:2006-05-20 Published:2006-05-20

PDF

1234

摘要： 目的建立兔肾脏缺血再灌注(I/R)损伤模型,通过肾动脉灌注移植自体骨髓干细胞,观察其对I/R损伤肾功能的影响。方法分离28只兔的骨髓干细胞,建立肾脏I/R损伤模型后随机均分为移植组和对照组。移植组于肾血流恢复后经肾动脉推注骨髓干细胞悬液,对照组同样方法推注等量生理盐水。分别于I/R损伤前和I/R损伤后第1、3、5、7、14、21、28天于兔耳缘静脉采血,检测血清肌酐(Scr)和尿素氮(BUN),观察肾功能情况,相同时间点取肾组织行病理学观察。结果I/R损伤后第1天和第3天两组动物Scr、BUN均达到最高水平,以后逐渐下降;第7天后移植组Scr及BUN水平逐渐低于对照组,到实验观察结束时的第28天,对照组Scr和BUN分别为(135.6±32.5)μmol/L和(10.9±2.5)mmol/L,移植组Scr和BUN分别为(90.1±11.1)μmol/L和(8.0±1.5)mmol/L,两组间有显著性差异(P<0.05)。病理学观察发现,I/R损伤后肾小管上皮细胞变性、坏死、脱落。结论骨髓干细胞移植能较早的降低肾脏I/R损伤后血清Scr和BUN水平,在一定程度上促进I/R损伤后肾功能修复。

Abstract: Objective To observe the effect of autologous bone marrow stem cell(BMSC)transplantation via the renal artery on renal function recovery following renal ischemic-reperfusion(I/R)injury in rabbits.Methods BMSCs were collected and isolated from rabbits.Twenty-eight rabbits were subjected to renal pedicle clamping for 105 min and randomized subsequently into transplantation group and control group.BMSCs or saline were injected into the kidney via the renal artery,respectively.Before and 1,3,5,7,14,21,and 28 days after I/R injury the venous blood was collected to measure the serum levels of SCr and BUN,and the renal tissue was sampled for pathological observation.Results One and 3 days after I/R injury,serum Cr and BUN levels increased significantly to the highest level in both groups.On the 7th day serum Cr and BUN levels in the transplantation group were lower than those in control group and remained so till the end of the experiment.On the 28th day,the levels of serum Cr(90.1±11.1 μmol/L)and BUN(8.0±1.5 mmol/L)in the transplantation group were significantly lower than those in the control group(135.6±32.5 μmol/L and 10.9±2.5 mmol/L,respectively,P<0.05).Pathological observation of the renal tissue revealed renal tubular epithelial cell degeneration,necrosis and abscission.Conclusion BMSC transplantation can accelerate renal function repair after acute tubular necrosis resulting from I/R injury,and decrease serum Cr and BUN levels in early stage following the injury. 

中图分类号: