南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (04): 476-478.

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急性分离的肺泡Ⅱ型细胞的钠转运及调节

申海燕； 李涛平；   

  1. 南方医科大学南方医院呼吸科； 南方医科大学南方医院呼吸科 广东 广州 510515； 广东 广州 510515；
  • 出版日期:2006-04-20 发布日期:2006-04-20
  • 基金资助:
    国家自然科学基金(30471720);广东省自然科学基金自由项目(04020458)~~

Sodium transport and its regulation of acutely isolated alveolar type Ⅱ cells

SHEN Hai-yan, LI Tao-ping Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China   

  1. 南方医科大学南方医院呼吸科； 南方医科大学南方医院呼吸科 广东 广州 510515； 广东 广州 510515；
  • Online:2006-04-20 Published:2006-04-20

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摘要： 目的观察急性分离的肺泡Ⅱ性细胞全细胞钠电流及调节特性。方法应用膜片钳技术全细胞记录模式记录分离后5h内的大鼠ATⅡ的全细胞钠电流,并观察阿米洛利和特布他林对其的影响。结果可以记录到阿米洛利敏感性钠电流,应用特布他林后电流明显增大。结论可以利用急性分离的ATⅡ细胞进行钠通道电生理研究。为病理状态下的研究开拓了新的视野。

Abstract: Objective To observe the sodium current of acutely isolated alveolar type Ⅱ cells and its regulation. Methods After isolation the AT Ⅱ cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect of amiloride and terbutaline on the current was investigated. Results The amiloride-sensitive current of AT Ⅱ cells were recorded, which could be obviously stimulated by terbutaline. Conclusion Acutely isolated AT Ⅱ cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions. 

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