南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (04): 463-.

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SARS冠状病毒S2基因变异性分析

周浩； 龙北国； 张文炳； 江丽芳； 陈丽丹； 贡树基； 赵卫；   

  1. 南方医科大学微生物教研室； 中山大学中山医学院微生物学教研室； 南方医科大学微生物教研室 广东 广州 510515； 广东 广州 510515； 广东 广州 510080；
  • 出版日期:2006-04-20 发布日期:2006-04-20
  • 基金资助:
    广东省科技攻关项目(A2004378)~~

Variability analysis of S2 gene of SARS-CoV

ZHOU Hao, LONG Bei-guo, ZHANG Wen-bing, JIANG Li-fang, CHEN Li-dan, GONG Shu-ji, ZHAO Wei Department of Microbiology, Southern Medical University, Guangzhou 510515, China; Department of Microbiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China   

  1. 南方医科大学微生物教研室； 中山大学中山医学院微生物学教研室； 南方医科大学微生物教研室 广东 广州 510515； 广东 广州 510515； 广东 广州 510080；
  • Online:2006-04-20 Published:2006-04-20

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摘要： 目的测定SARS冠状病毒GD322株S2基因序列并对SARS-CoV S2基因进行系统进化树分析。方法用RT-PCR 法从SARS-CoV GD322株基因组中扩增S2基因片段并克隆至pGEM-T载体,转化大肠杆菌DH5α株后测序。利用 Lasergene、ClustalX、DNAman和Treeview等软件,将基因序列翻译成氨基酸序列后,与早、中、晚期各地暴发的SARS-CoV S2蛋白序列进行比对,构建系统进化树,并在Internet网数据库中对S2蛋白氨基酸序列进行T细胞抗原表位预测。结果随着SARS-CoV的传播流行,S2基因趋于稳定,晚期的SARS-CoV S2基因的同源性达到99．9％。T细胞抗原表位预测发现,SARS-CoV S2蛋白第57位氨基酸突变对T细胞抗原表位有影响。结论 SARS流行过程中SARS-CoV S2 基因较为稳定,S2蛋白57位氨基酸的突变可影响病毒T细胞抗原表位。

Abstract: Objective To determine the sequence of S2 gene of SARS-associated coronavirus (SARS-CoV) GD322 and analyze the phyletic evolution of S2 gene. Method S2 gene fragment was amplified from SARS-CoV GD322 genome with RT-PCR and ligated to pGEM-T vector for sequence analysis after transformation of the plasmid into E.coli DH5a. The variability of S2 genes and S2 proteins from 12 strains isolated in the early, intermediate and advanced stages of the SARS outbreak were analyzed and the phylogenetic tree was constructed with Lasergene, Clustal X, DNAman and Treeview. T cell antigen epitopes of S2 protein were predicted on the basis of Internet database. Result With the epidemic spread of SARS-CoV, the S2 genes of the virus tended to become stable. Homology of S2 genes of SARS-CoV isolated in advanced stage of the outbreak reached 99.9%. Prediction of T cell antigen epitope showed that mutation at the 57th amino acid effected T cell antigen epitope. Conclusion S2 gene of GD322 SARS-CoV is relatively stable during the epidemic spread of the virus, and mutation at the 57th amino acids of S2 protein may affect the T cell antigen epitope.

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