南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (04): 441-444.

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STAT3在食管鳞癌细胞系中的激活及其靶基因产物的表达

王新华； 李珊珊； 阎爱华； 卢创新； 郭燕萍；   

  1. 郑州大学第一附属医院病理科／河南省肿瘤病理重点实验室； 郑州大学基础医学院； 郑州大学第一附属医院病理科／河南省肿瘤病理重点实验室 第三附属医院儿内科； 河南 郑州 450052；
  • 出版日期:2006-04-20 发布日期:2006-04-20
  • 基金资助:
    “十五”“211工程”重点学科建设项目教重办(2002)第2号;河南省科技攻关基金(0424410033)~~

Constitutive activation of signal transducers and activators of transcription 3 and expression of its target gene products in human ESCC cell line

WANG Xin-hua, LI Shan-shan, YAN Ai-hua, LU Chuang-xin, GUO Yan-ping Key Laboratory of Tumor Pathology of Henan Province/Department of Pathology, First Affiliated Hospital, Department of Pediatrics, Third Affiliated Hospital, Department of Precancerous Study, College of Basic Medical Science, Zhengzhou University, Zhengzhou 450052, China   

  1. 郑州大学第一附属医院病理科／河南省肿瘤病理重点实验室； 郑州大学基础医学院； 郑州大学第一附属医院病理科／河南省肿瘤病理重点实验室 第三附属医院儿内科； 河南 郑州 450052；
  • Online:2006-04-20 Published:2006-04-20

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摘要： 目的分析食管鳞癌细胞系中信号传导及转录活化因子3(STAT3)在不同食管鳞癌细胞系中的激活状态及其对食管鳞癌细胞的生存及转化作用,探讨是否在不同类型的食管鳞癌细胞系中存在组成性激活的STAT3信号通路。方法采用Western blotting检测两株食管鳞癌细胞中STAT3及磷酸化STAT3(p-STAT3),血管内皮生长因子(VEGF)和 BCl-2蛋白的表达情况,并采用凝胶电泳迁移率(EMSA)法检测两株食管鳞癌细胞核中p-STAT3蛋白与其特异的 DNA序列结合能力。RT-PCR法检测STAT3、VEGF7和BCl-2 mRNA的表达。结果食管鳞癌细胞中存在激活的STAT3 信号传导通路,通路蛋白STAT3、p-STAT3及其下游靶基因产物VEGF、BCl-2在两种食管鳞癌细胞中均有表达, p-STAT3蛋白在细胞核中具有较高的DNA结合活性。RT-PCR结果表明,STAT3、VEGF和BCl-2的mRNA在细胞中也存在过表达。结论本研究发现在两种食管鳞癌细胞系中均有组成性激活的STAT3信号通路,提示STAT3信号通路可能在食管鳞癌发生、发展中起重要作用。

Abstract: Objective To investigate the expression of signal transducers and activators of transcription 3 (STAT3) and its target gene products including vascular endothelial growth factor (VEGF) and Bcl-2 in two human esophageal squamous cell carcinoma (ESCC) cell lines for understanding whether STAT3 signaling transduction pathway was constitutively activated in ESCC cell lines. Methods Western blotting was used to determine the protein levels of STAT3, p-STAT3 (activated STAT3), VEGF and Bcl-2 in two ESCC cell lines (EC9706 and Eca109). Nuclear proteins from the ESCC cell lines were extracted to evaluate the DNA-binding activity by electrophoretic mobility gel shift assay (EMSA). RT-PCR was used to detect the mRNA of STAT3, VEGF and Bcl-2. Results Western blotting and RT-PCR revealed that STAT3, VEGF and Bcl-2 protein and mRNA were ovexpressed in the two ESCC cell lines, which contained constitutively activated STAT3 signaling transduction pathway. The results of EMSA of the nuclear protein showed high DNA-binding activity of STAT3. Conclusion STAT3 signaling transduction pathway is constitutively activated in human ESCC cell lines, suggesting that STAT3 may play a critical role in the carcinogenesis of the esophagus. 更多

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