南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (04): 381-385.

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T-2毒素对软骨Ⅱ型胶原蛋白和蛋白聚糖合成的影响以及硒的保护作用

陈静宏； 曹峻岭； 楚雍烈； 杨占田； 师钟丽； 王红林； 郭雄； 王治伦；   

  1. 西安交通大学地方病研究所及环境与疾病相关性基因教育部重点实验室和微量元素与地方病卫生部重点实验室； 西安交通大学医学院病原微生物教研室； 西安交通大学地方病研究所及环境与疾病相关性基因教育部重点实验室和微量元素与地方病卫生部重点实验室 陕西 西安 710061；
  • 出版日期:2006-04-20 发布日期:2006-04-20
  • 基金资助:
    教育部科技重点项目(03152);国家自然科学基金(30471499)~~

Protective effect of selenium against T-2 toxin-induced inhibition of chondrocyte aggrecan and collagen Ⅱ synthesis

CHEN Jing-hong, CAO Jun-ling, CHU Yong-lie, YANG Zhan-tian, SHI Zhong-li, WANG Hong-lin, GUO Xiong, WANG Zhi-lun Institute of Endemic Diseases, Medical School of Xi’an Jiaotong University/Key Laboratory of Environment and Disease-related Genes of Education Ministr;Department of Microbiology, Medical School of Xi’an Jiaotong University, Xi’an 710061, China   

  1. 西安交通大学地方病研究所及环境与疾病相关性基因教育部重点实验室和微量元素与地方病卫生部重点实验室； 西安交通大学医学院病原微生物教研室； 西安交通大学地方病研究所及环境与疾病相关性基因教育部重点实验室和微量元素与地方病卫生部重点实验室 陕西 西安 710061；
  • Online:2006-04-20 Published:2006-04-20

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摘要： 目的探讨T-2毒素对软骨细胞主要间质成分Ⅱ型胶原蛋白和蛋白聚糖合成的影响和硒的保护作用。方法采用 MTT法检测T-2毒素对软骨细胞存活率的影响;用TB染色法检测人软骨细胞蛋白聚糖表达;免疫组织化学检测人软骨细胞胶原蛋白Ⅱ表达;RT-PCR检测人软骨细胞Ⅱ型胶原蛋白和蛋白聚糖mRNA表达。结果 T-2毒素在浓度为 0．001-2 mg／L的范围内,对软骨细胞的作用呈较典型的浓度依赖关系和时间依赖关系,而且随着作用时间的延长,浓度依赖关系更加明显。T-2毒素(10～20μg／L)可以抑制软骨细胞Ⅱ型胶原蛋白和蛋白聚糖的合成及其mRNA表达。而加硒能较明显刺激软骨细胞蛋白聚糖mRNA表达,部分减弱T-2毒素的这种抑制,有一定的保护作用,但不显示对T-2 毒素引起的Ⅱ型胶原蛋白及其mRNA表达的保护作用。结论 T-2毒素对软骨细胞Ⅱ型胶原蛋白和蛋白聚糖合成有明显的抑制作用,微量元素硒对T-2毒素引起的软骨细胞蛋白聚糖改变有一定保护作用。 更多还原

Abstract: Objective To study the inhibitory effect of T-2 toxin on the expression of aggrecan and collagen Ⅱ in chondrocytes and the protection of selenium against this effect. Methods Human chondrocytes cultured in vitro were treated with T-2 toxin at different concentrations for varied time periods (1-5 days), and the cell viability was measured by MTT assay. Aggrecan expression was detected by toluidine blue staining and collagen Ⅱ expression by immunostaining using monoclonal antibody of collagen. Aggrecan and collagen Ⅱ mRNA expressions were measured by semiquantitative RT-PCR. Results T-2 toxin dose- and time-dependently affected chondrocyte viability within the concentration range of 0.001-2 mg/L, the prolonged treatment time further enhanced the dose dependence of the inhibitory effect. T-2 toxin lowered aggrecan and collagen Ⅱ synthesis in the chondrocytes and reduced their mRNA expressions. Selenium could partly attenuate the inhibitory effects of T-2 toxin on aggrecan mRNA expression, but showed no such effect against T-2-induced collagen Ⅱ expression. Conclusion T-2 toxin can obviously inhibit aggrecan and collagen Ⅱ synthesis in human chondrocytes, and selenium can partly antagonize the inhibitory effects of T-2 toxin on aggrecan. 

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