血卟啉-赫赛汀光敏免疫偶联物的制备及光敏免疫疗法研究

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (03): 355-357.

血卟啉-赫赛汀光敏免疫偶联物的制备及光敏免疫疗法研究

陈璐；罗荣城；李黎波；严晓；丁雪梅；

南方医科大学南方医院肿瘤中心；南方医科大学南方医院肿瘤中心广东广州510515；广东广州510515；

出版日期:2006-03-20 发布日期:2006-03-20

Preparation of hematoporphyrin-herceptin photoimmunogonjucate for photoimmunotherapy

CHEN Lu,LUO Rong-cheng,LI Li-bo,YAN Xiao,DING Xue-mei Center of Oncology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China

南方医科大学南方医院肿瘤中心；南方医科大学南方医院肿瘤中心广东广州510515；广东广州510515；

Online:2006-03-20 Published:2006-03-20

摘要/Abstract

摘要： 目的制备光敏剂血卟啉(HP)-单克隆抗体赫赛汀的光敏免疫偶联物,并观察偶联物对BT-474人乳腺癌细胞的杀伤及凋亡作用。方法以碳二亚胺为缩合剂,化学制备HP-赫赛汀光敏免疫偶联物。给予波长630 nm激光照射后,用MTT法、FCM检测比较免疫偶联物和游离HP对BT-474人乳腺癌细胞的杀伤及凋亡作用。结果含同剂量HP的偶联物较游离HP对BT-474细胞的杀伤及凋亡效果明显增强(P<0.05)。结论成功制备HP-赫赛汀光敏免疫偶联物,该偶联物对BT-474人乳腺癌细胞的杀伤作用强于游离HP。更多还原

Abstract: Objective To prepare photoimmunoconjugate of hematoporphyrin(HP) and herceptin,and study its killing and apoptosis-inducing effect on tumor cells BT-474.Methods HP-herceptin photoimmunoconjugate was synthesized with EDCI as the condensator.After exposure of the cells to 630 nm laser,the killing effect of the conjugate and cell apoptosis were evaluated by MTT assay and flow cytometry.Results Compared with free HP at equivalent dose,the immune reactivity,killing effect and the apoptosis-inducing effect of HP-herceptin immunoconjugate on BT-474 cells was enhanced(P<0.05).Conclusion The killing effect of HP-herceptin immunoconjugate is stronger than free HP on BT-474 cells.

中图分类号:

R943
R73-36

陈璐；罗荣城；李黎波；严晓；丁雪梅； . 血卟啉-赫赛汀光敏免疫偶联物的制备及光敏免疫疗法研究[J]. 南方医科大学学报, 2006, 26(03): 355-357.

CHEN Lu,LUO Rong-cheng,LI Li-bo,YAN Xiao,DING Xue-mei Center of Oncology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China. Preparation of hematoporphyrin-herceptin photoimmunogonjucate for photoimmunotherapy[J]. Journal of Southern Medical University, 2006, 26(03): 355-357.