南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (03): 348-351.

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大鼠脑外伤后P-selectin表达的变化及意义

张荣军； 游潮； 蔡博文； 贺民； 杨咏波； 刘展； 焦庆芳；   

  1. 四川大学华西医院神经外科； 四川大学华西医院神经外科 四川成都610041； 四川成都610041；
  • 出版日期:2006-03-20 发布日期:2006-03-20

Changes in P-selectin expression after brain injury in rats

ZHANG Rong-jun,YOU Chao,CAI Bo-wen,HE Min,YANG Yong-bo,LIU Zhan,JIAO Qing-fang Department of Neurosurgery,West China Hospital,Sichuan University,Chengdu 610041,China   

  1. 四川大学华西医院神经外科； 四川大学华西医院神经外科 四川成都610041； 四川成都610041；
  • Online:2006-03-20 Published:2006-03-20

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摘要： 目的探讨大鼠脑外伤后P-selectin的表达变化及其与脑水肿等继发性脑损伤的关系。方法采用Feeney大鼠脑损伤动物模型,将60只Sprague-Dawley大鼠随机分为3组:A(标准对照组),B(轻伤组)、C组(重伤组),每组20只。分别于脑外伤后6 h、24 h、3 d、7 d随机各取标本5只,测量脑水肿程度以及P-selectin在脑组织的表达变化以及免疫阳性染色血管,并进行计算机图像分析系统和统计学分析。结果外伤后6 h P-selectin的表达及粒细胞在脑组织的浸润显著升高(P<0.05),于伤后24 h达到高峰,然后逐渐降低。结论脑外伤后P-selectin的表达及粒细胞在脑组织的浸润显著升高,其时程和空间分布与脑外伤后继发性损伤相吻合。提示P-selectin的表达增加可能参与了脑外伤后的继发性损伤。 更多还原

Abstract: Objective To explore the relationship between expression thange of P-selectin after brain injury and secondary brain damage.Methods Sixty SD rats were randomized into 3 equal groups,namely the control group,mild injury group and severe injury group and animal models of brain injury were established in SD rats according to the method of Feeney.P-selectin expression in the brain tissues were determined at 6 h and l,3,and 7 days following brain injury(n=5 for each time point).Imaging analysis was performed using computerized imaging technique.Results P-selectin expression and neutrophil infiltration in the brain tissues increased significantly 6 h after brain injury(P<0.05),reaching the peak level at postoperative 24 h and then gradually decreased.Conclusion P-selectin expression and neutrophil infiltration increase significantly following brain injury,and the time course and distribution of P-selectin expression are consistent with the secondary damage of the brain,strongly suggesting the involvement of P-selectin upregulation in the secondary insult after brain injury.

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