南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (03): 284-.

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广州管圆线虫病疗效考核抗原的筛选与鉴定

赵醒村； 顾金保； 李华； 刘敏； 沈浩贤； 陈晓光；   

  1. 南方医科大学病原生物学系； 广州医学院病原生物学教研室； 南方医科大学病原生物学系 广东广州510515； 广东广州510515； 广东广州510182；
  • 出版日期:2006-03-20 发布日期:2006-03-20
  • 基金资助:
    国家科技部十五重大科技攻关项目(2003BA712A03-07)~~

Screening and idetification of therapeutic effect evaluation antigens of angiostrongyliasis

ZHAO Xing-cun1,GU Jin-bao1,LI Hua1,LIU Min1,SHEN Hao-xian2,CHEN Xiao-guang1 1Department of Pathogenic Biology,Southern Medical University,Guangzhou 510515,China;2Department of Pathogenic Biology,Guangzhou Medical College,Guangzhou 510182,China   

  1. 南方医科大学病原生物学系； 广州医学院病原生物学教研室； 南方医科大学病原生物学系 广东广州510515； 广东广州510515； 广东广州510182；
  • Online:2006-03-20 Published:2006-03-20

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摘要： 目的从广州管圆线虫抗原中筛选广州管圆线虫病疗效考核抗原。方法用治疗前后广州管圆线虫感染的大鼠血清与广州管圆线虫可溶性抗原进行免疫印迹试验,用ELISA检测治疗前及治疗后不同时期感染大鼠血清中的AC-IgG和AC32-IgG抗体。结果Mr 38 000￣78 000区间的抗原分子与治疗前后的感染大鼠血清的反应带无明显差别,Mr 190 000和Mr 17 000抗原与治疗前大鼠血清出现较强的反应,与治疗后血清未出现反应带,Mr 32 000和Mr 24 000抗原与治疗前大鼠血清出现较强的反应,与治疗后大鼠血清的反应带明显变弱;AC32-IgG抗体出现的高峰期较早,而且在治疗后消失较快,治疗后17周的阴转率为60%(6/10)。结论广州管圆线虫Mr 190 000,17 000,32 000,24 000,16 000抗原具有潜在的疗效考核价值,ELISA检测AC32-IgG抗体可以用于感染大鼠的疗效考核。 更多还原

Abstract: Objective To identify antigens which may help evaluate the therapeutic effect of angiostrongyliasis from adult worm antigen of Angiostrongylus cantonensis.Methods The adult worm antigens of A.cantonensis were analyzed by Western blotting with the sera of rats infected with A.cantonensis before and after treatment.The sera of rats were tested by enzyme-linked immunosorbent assay(ELISA).Results The antigens with relative molecular mass between 38 000 and 78 000 reacted not only with the sera of rats before treatment,but also with that after treatment.The antigens with Mr between 190 000 and 17 000 reacted with the sera of rats before treatment but not with that after treatment;those with Mr between 32 000 and 24 000 antigens strongly reacted with the former,but the reaction became much weakened with the latter.The AC32-IgG antibody appeared earlier than the AC-IgG,and disappeared rapidly after treatment.Six of the 10 treated rats became negative for AC-IgG as found by ELISA.Conclusion The antigens of adult worm antigen of A.cantonensis with Mr of 190 000,32 000,24 000,17 000 and 16 000 may serve as candidate antigens for therapeutic effect evaluation of angiostrongyliasis.

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