南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (03): 266-269.

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阻断PLC-γ1通路对TNF-α抑制胶质瘤细胞增殖并诱导其凋亡的影响

林骏； 杨进城； 谭丽； 罗深秋；   

  1. 南方医科大学细胞生物学教研室； 南方医科大学细胞生物学教研室 广东广州510515； 广东广州510515；
  • 出版日期:2006-03-20 发布日期:2006-03-20
  • 基金资助:
    国家自然科学基金项目(39870381);广东省自然科学基金项目(020015);广东省自然科学基金重点项目(05102580)~~

Sensitization of human glioma SWO cell line to tumor necrosis factor-induced apoptosis by blocking phospholipase C-γ1 signaling pathway

LIN Jun,YANG Jin-Cheng,TAN Li,LUO Shen-Qiu Department of Cell Biology,Southern Medical University,Guangzhou 510515,China   

  1. 南方医科大学细胞生物学教研室； 南方医科大学细胞生物学教研室 广东广州510515； 广东广州510515；
  • Online:2006-03-20 Published:2006-03-20

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摘要： 目的探讨磷脂酶C-γ1(PLC-γ1)在肿瘤坏死因子-α(TNF-α)诱导胶质瘤细胞凋亡中的作用。方法应用PLC-γ1的特异性抑制剂U73122抑制SWO胶质瘤细胞PLC-γ1活性,用MTT法观察在阻断PLC-γ1通路前后,TNF-α对SWO胶质瘤细胞的增殖抑制作用;流式细胞仪检测TNF-α诱导SWO胶质瘤细胞凋亡的情况;Western免疫印记法检测TNF-α是否激活caspase-3以及抑凋亡蛋白bcl-2表达的情况。结果抑制PLC-γ1活性后,SWO胶质瘤细胞对低浓度TNF-α的敏感性显著增加。结论阻断PLC-γ1通路本身虽不能直接启动凋亡,但却能增加SWO胶质瘤细胞对某些调亡因素(如TNF-α)的敏感性,其分子机制之一可能是下调抑凋亡基因bcl-2的表达。 更多还原

Abstract: Objective To explore the role of phospholipase C-γ1(PLC-γ1) in tumor necrosis factor-α(TNF-α)-induced apoptosis of human glioma SWO cells.Methods The PLC-γ1 pathway was blocked by U73122 in SWO cells,and the inhibitory effect of TNF-α on SWO glioma cell proliferation with or without U73122 treatment was investigated by MTT assay.The cell apoptosis induced by TNF-α along or in combination with U73122 was detected by flow cytometry with PI staining.The expression of caspase-3 and Bcl-2 was detected by Western blotting.Results and Conclusion U73122 can sensitize SWO glioma cells to TNF-α-induced apoptosis.Blocking the PLC-γ1 pathway may not induce apoptosis of SWO glioma cells,but can sensitize SWO glioma cells to small-dose TNF-α-induced apoptosis,the mechanism of which may involve down-regulation of bcl-2.

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