南方医科大学学报

南方医科大学学报 ›› 2006, Vol. 26 ›› Issue (03): 251-254.

• •    下一篇

Survivin siRNA协同5-FU抑制MCF-7细胞的增殖(英文)

张淑群； 张淑慧； 薛兴欢； 王西京； 姜建涛；   

  1. 西安交通大学第二医院肿瘤外科； 西北工业大学化学工程系； 西安交通大学第二医院肿瘤外科 陕西西安710004； 陕西西安710072； 陕西西安710004；
  • 出版日期:2006-03-20 发布日期:2006-03-20
  • 基金资助:
    Supported by National Natural Science Foundation of China(30500600)and Key Sci-Tech Research Project of Shaanxi Province(2003k10-G41)

Synergism between a siRNA targeted to survivin and 5-FU in inhibiting MCF-7 cell proliferation in vitro

ZHANG Shu-qun1,ZHANG Shu-hui2,XUE Xin-huan1,WANG Xi-jing1,JIANG Jian-tao1 1Department of Oncology,Second Hospital,Xi’an Jiaotong University,Xi’an 710004,China;2Department of Chemical Engineering,Northwestern Polytechnical University,Xi’an 710072,China   

  1. 西安交通大学第二医院肿瘤外科； 西北工业大学化学工程系； 西安交通大学第二医院肿瘤外科 陕西西安710004； 陕西西安710072； 陕西西安710004；
  • Online:2006-03-20 Published:2006-03-20

PDF

1113

摘要： 目的研究以survivin为靶标的小干扰RNA(siRNA)与化疗药5-FU联合应用抑制MCF-7细胞增殖的作用。方法利用T7 RNA聚合酶体外转录合成siRNA并筛选出一条RNA干扰survivin基因的靶序列。以脂质体为载体,将survivin siRNA转染至人乳腺癌MCF-7细胞中,用四氮唑盐(MTT)法染色并计算siRNA联用5-FU对MCF-7细胞的抑制率,用SAS统计软件及金正均Q值法进行统计分析。结果单用5-FU处理细胞,其IC50为4.42μg/ml,而加入5 nmol/L siRNA后,其IC50降为1.18μg/ml,siRNA与5-FU联用对MCF-7细胞的抑制作用较单用5-FU明显增强(F=26.74,P<0.01);Q值分析表明survivin siRNA与中低浓度的5-FU联用,有较好的协同作用(Q≥1.15)。结论survivinsiRNA可显著增强5-FU对MCF-7细胞增殖的抑制,提高肿瘤细胞对化疗药物的敏感性,克服耐药性的产生。 更多还原

Abstract: Objective To investigate the role of small interfering RNA(siRNA) targeted to survivin in combination with 5-fluorouracil(5-FU) in inhibiting the proliferation of MCF-7 cells.Methods A siRNA targeted to survivin was synthesized and transfected into MCF-7 cells via lipofectin.Changes of the cell growth activity in response to combined treatment with survivin siRNA and 5-FU or 5-FU treatment alone was evaluated by MTT assay.The Q method of Jin Zhenjun was used to evaluated synergism between the synthesized siRNA and 5-FU.Results Treatment with 5 nmol/L siRNA reduced the IC50 of 5-FU from 4.42 to 1.18 μg/ml,and the inhibitory effect of combined treatment on MCF-7 cells was higher than that of 5-FU alone(F=26.74,P<0.01).Synergism effect(Q≥1.15) was observed between 5-FU at lower concentrations and survivin siRNA.Conclusion siRNA may enhance the effectiveness of 5-FU in inhibiting the proliferation of MCF-7 cells.

中图分类号: